Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
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| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| GSK1059615 | MCF 10A | 10.0 | uM | 6 | hr | 225.75 | 115.01 | p-Akt | 12.62 | |
| GSK1059615 | MCF 10A | 10.0 | uM | 24 | hr | 200.07 | 77.09 | p-Akt | 13.27 | |
| MK2206 | MCF 10A | 10.0 | uM | 6 | hr | 336.78 | 130.66 | p-Akt | 15.48 | |
| MK2206 | MCF 10A | 10.0 | uM | 24 | hr | 310.25 | 82.81 | p-Akt | 12.52 | |
| Triciribine | MCF 10A | 10.0 | uM | 6 | hr | 337.64 | 131.68 | p-Akt | 15.51 | |
| Triciribine | MCF 10A | 10.0 | uM | 24 | hr | 284.66 | 92.69 | p-Akt | 18.74 | |
| Dactolisib | MCF 10A | 10.0 | uM | 6 | hr | 277.17 | 129.83 | p-Akt | 14.88 | |
| Dactolisib | MCF 10A | 10.0 | uM | 24 | hr | 395.02 | 66.41 | p-Akt | 17.83 | |
| Gefitinib | T47D | 10.0 | uM | 6 | hr | 157.55 | 89.42 | p-Akt | 106.87 | |
| Gefitinib | T47D | 10.0 | uM | 24 | hr | 139.09 | 65.73 | p-Akt | 66.96 | |
| Torkinib | T47D | 10.0 | uM | 6 | hr | 152.43 | 69.07 | p-Akt | 14.87 | |
| Torkinib | T47D | 10.0 | uM | 24 | hr | 137.62 | 40.90 | p-Akt | 14.21 | |
| GSK1059615 | T47D | 10.0 | uM | 6 | hr | 189.09 | 66.04 | p-Akt | 16.13 | |
| GSK1059615 | T47D | 10.0 | uM | 24 | hr | 190.43 | 66.91 | p-Akt | 17.57 | |
| MK2206 | T47D | 10.0 | uM | 6 | hr | 110.32 | 28.55 | p-Akt | 6.57 | |
| MK2206 | T47D | 10.0 | uM | 24 | hr | 188.01 | 51.80 | p-Akt | 14.95 | |
| Triciribine | T47D | 10.0 | uM | 6 | hr | 110.90 | 45.64 | p-Akt | 9.31 | |
| Triciribine | T47D | 10.0 | uM | 24 | hr | 179.90 | 57.17 | p-Akt | 33.20 | |
| Dactolisib | T47D | 10.0 | uM | 6 | hr | 93.15 | 46.29 | p-Akt | 6.63 | |
| Dactolisib | T47D | 10.0 | uM | 24 | hr | 153.18 | 108.52 | p-Akt | 16.83 | |
| Gefitinib | AU565 | 0 (control) | 6 | hr | 239.03 | 48.95 | p-4EBP1 | 49.90 | | |
| Gefitinib | AU565 | 0 (control) | 24 | hr | 567.06 | 138.57 | p-4EBP1 | 168.25 | | |
| Torkinib | AU565 | 0 (control) | 6 | hr | 240.39 | 56.13 | p-4EBP1 | 67.78 | | |
| Torkinib | AU565 | 0 (control) | 24 | hr | 470.66 | 130.67 | p-4EBP1 | 221.68 | | |
| GSK1059615 | AU565 | 0 (control) | 6 | hr | 212.15 | 50.86 | p-4EBP1 | 60.63 | |