Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
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| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| Torkinib | MCF 10A | 1.78 | nM | 6 | hr | 433.78 | 166.62 | p-4EBP1 | 324.13 | |
| Torkinib | MCF 10A | 1.78 | nM | 24 | hr | 364.66 | 163.44 | p-4EBP1 | 234.28 | |
| GSK1059615 | MCF 10A | 10.0 | nM | 6 | hr | 401.96 | 171.56 | p-4EBP1 | 355.01 | |
| GSK1059615 | MCF 10A | 10.0 | nM | 24 | hr | 279.03 | 145.93 | p-4EBP1 | 268.60 | |
| MK2206 | MCF 10A | 10.0 | nM | 6 | hr | 386.97 | 157.19 | p-4EBP1 | 348.64 | |
| MK2206 | MCF 10A | 10.0 | nM | 24 | hr | 288.54 | 161.95 | p-4EBP1 | 241.30 | |
| Triciribine | MCF 10A | 10.0 | nM | 6 | hr | 388.83 | 158.76 | p-4EBP1 | 350.13 | |
| Triciribine | MCF 10A | 10.0 | nM | 24 | hr | 249.12 | 152.71 | p-4EBP1 | 219.19 | |
| Dactolisib | MCF 10A | 10.0 | nM | 6 | hr | 347.50 | 141.22 | p-4EBP1 | 316.53 | |
| Dactolisib | MCF 10A | 10.0 | nM | 24 | hr | 248.03 | 157.81 | p-4EBP1 | 175.70 | |
| Gefitinib | T47D | 10.0 | nM | 6 | hr | 175.71 | 99.57 | p-4EBP1 | 491.96 | |
| Gefitinib | T47D | 10.0 | nM | 24 | hr | 158.85 | 126.81 | p-4EBP1 | 425.94 | |
| Torkinib | T47D | 1.78 | nM | 6 | hr | 167.95 | 89.40 | p-4EBP1 | 486.38 | |
| Torkinib | T47D | 1.78 | nM | 24 | hr | 164.91 | 104.23 | p-4EBP1 | 387.34 | |
| GSK1059615 | T47D | 10.0 | nM | 6 | hr | 168.20 | 86.24 | p-4EBP1 | 489.92 | |
| GSK1059615 | T47D | 10.0 | nM | 24 | hr | 150.30 | 110.80 | p-4EBP1 | 419.73 | |
| MK2206 | T47D | 10.0 | nM | 6 | hr | 91.10 | 36.69 | p-4EBP1 | 83.19 | |
| MK2206 | T47D | 10.0 | nM | 24 | hr | 140.94 | 108.00 | p-4EBP1 | 379.38 | |
| Triciribine | T47D | 10.0 | nM | 6 | hr | 101.35 | 56.34 | p-4EBP1 | 175.96 | |
| Triciribine | T47D | 10.0 | nM | 24 | hr | 149.41 | 107.99 | p-4EBP1 | 375.92 | |
| Dactolisib | T47D | 10.0 | nM | 6 | hr | 93.60 | 47.20 | p-4EBP1 | 138.33 | |
| Dactolisib | T47D | 10.0 | nM | 24 | hr | 129.25 | 89.70 | p-4EBP1 | 315.12 | |
| Gefitinib | AU565 | 31.6 | nM | 6 | hr | 209.97 | 51.77 | p-4EBP1 | 55.32 | |
| Gefitinib | AU565 | 31.6 | nM | 24 | hr | 517.21 | 129.55 | p-4EBP1 | 150.66 | |
| Torkinib | AU565 | 7.5 | nM | 6 | hr | 237.10 | 47.79 | p-4EBP1 | 50.80 | |