Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
WM-115 Vemurafenib Rocilinostat 1.0000 0.1067
uM
 48
hr
 2 1382 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 138.42 NGFR rabbit IgG(H+L) / donkey NGFR 1083.28
WM115-B2-rep2
B5
WM-115 Vemurafenib GSK-J1 1.0000 0.1067
uM
 48
hr
 2 1158 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 143.88 NGFR rabbit IgG(H+L) / donkey NGFR 1182.56
WM115-B2-rep2
B6
WM-115 Vemurafenib PFI-1 1.0000 0.1067
uM
 48
hr
 2 1186 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 141.71 NGFR rabbit IgG(H+L) / donkey NGFR 1098.16
WM115-B2-rep2
B7
WM-115 Vemurafenib UNC669 1.0000 0.1067
uM
 48
hr
 2 1142 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 138.65 NGFR rabbit IgG(H+L) / donkey NGFR 1180.01
WM115-B2-rep2
B8
WM-115 Vemurafenib Veliparib 1.0000 0.1067
uM
 48
hr
 2 1355 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 139.84 NGFR rabbit IgG(H+L) / donkey NGFR 1032.08
WM115-B2-rep2
C2
WM-115 Vemurafenib Rucaparib 1.0000 0.1067
uM
 48
hr
 2 1286 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 135.71 NGFR rabbit IgG(H+L) / donkey NGFR 1110.95
WM115-B2-rep2
C3
WM-115 Vemurafenib Belinostat 1.0000 0.1067
uM
 48
hr
 2 1389 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 130.46 NGFR rabbit IgG(H+L) / donkey NGFR 499.87
WM115-B2-rep2
C4
WM-115 Vemurafenib Selisistat 1.0000 0.1067
uM
 48
hr
 2 1468 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 140.56 NGFR rabbit IgG(H+L) / donkey NGFR 1089.03
WM115-B2-rep2
C5
WM-115 Vemurafenib GSK-J2 1.0000 0.1067
uM
 48
hr
 2 1417 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 135.53 NGFR rabbit IgG(H+L) / donkey NGFR 1067.45
WM115-B2-rep2
C6
WM-115 Vemurafenib (+)-JQ1 1.0000 0.1067
uM
 48
hr
 2 1160 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 129.96 NGFR rabbit IgG(H+L) / donkey NGFR 695.15
WM115-B2-rep2
C7
WM-115 Vemurafenib UNC1215 1.0000 0.1067
uM
 48
hr
 2 1460 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 129.83 NGFR rabbit IgG(H+L) / donkey NGFR 1111.05
WM115-B2-rep2
C8
WM-115 Vemurafenib Vorinostat 1.0000 0.1067
uM
 48
hr
 2 1513 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 138.96 NGFR rabbit IgG(H+L) / donkey NGFR 1082.99
WM115-B2-rep2
D2
WM-115 Vemurafenib JW55 1.0000 0.1067
uM
 48
hr
 2 1482 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 140.32 NGFR rabbit IgG(H+L) / donkey NGFR 1155.40
WM115-B2-rep2
D3
WM-115 Vemurafenib Entinostat 1.0000 0.1067
uM
 48
hr
 2 1807 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 137.38 NGFR rabbit IgG(H+L) / donkey NGFR 1494.32
WM115-B2-rep2
D4
WM-115 Vemurafenib AGK2 1.0000 0.1067
uM
 48
hr
 2 1468 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 123.46 NGFR rabbit IgG(H+L) / donkey NGFR 1223.13
WM115-B2-rep2
D5
WM-115 Vemurafenib GSK-J4 1.0000 0.1067
uM
 48
hr
 2 1662 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 153.28 NGFR rabbit IgG(H+L) / donkey NGFR 1193.23
WM115-B2-rep2
D6
WM-115 Vemurafenib (-)-JQ1 1.0000 0.1067
uM
 48
hr
 2 1607 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 138.27 NGFR rabbit IgG(H+L) / donkey NGFR 1139.85
WM115-B2-rep2
D7
WM-115 Vemurafenib IOX2 1.0000 0.1067
uM
 48
hr
 2 1530 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 136.30 NGFR rabbit IgG(H+L) / donkey NGFR 1179.71
WM115-B2-rep2
D8
WM-115 Vemurafenib Azacitidine 1.0000 0.1067
uM
 48
hr
 2 1410 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 142.24 NGFR rabbit IgG(H+L) / donkey NGFR 1072.40
WM115-B2-rep2
E2
WM-115 Vemurafenib C646 1.0000 0.1067
uM
 48
hr
 2 1309 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 158.79 NGFR rabbit IgG(H+L) / donkey NGFR 1107.75
WM115-B2-rep2
E3
WM-115 Vemurafenib Mocetinostat 1.0000 0.1067
uM
 48
hr
 2 1572 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 120.44 NGFR rabbit IgG(H+L) / donkey NGFR 1493.64
WM115-B2-rep2
E4
WM-115 Vemurafenib Resveratrol 1.0000 0.1067
uM
 48
hr
 2 1381 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 140.09 NGFR rabbit IgG(H+L) / donkey NGFR 1087.55
WM115-B2-rep2
E5
WM-115 Vemurafenib Daminozide 1.0000 0.1067
uM
 48
hr
 2 1438 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 132.87 NGFR rabbit IgG(H+L) / donkey NGFR 1174.36
WM115-B2-rep2
E6
WM-115 Vemurafenib I-BET 1.0000 0.1067
uM
 48
hr
 2 1425 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 136.30 NGFR rabbit IgG(H+L) / donkey NGFR 924.32
WM115-B2-rep2
E7
WM-115 Vemurafenib Epigallocatechin gallate 1.0000 0.1067
uM
 48
hr
 2 1519 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 126.36 NGFR rabbit IgG(H+L) / donkey NGFR 1180.29
WM115-B2-rep2
E8