Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
WM-115 Vemurafenib Decitabine 1.0000 0.1067
uM
 48
hr
 2 1393 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 141.04 NGFR rabbit IgG(H+L) / donkey NGFR 1121.73
WM115-B2-rep2
F2
WM-115 Vemurafenib Garcinol 1.0000 0.1067
uM
 48
hr
 2 1397 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 141.18 NGFR rabbit IgG(H+L) / donkey NGFR 1141.90
WM115-B2-rep2
F3
WM-115 Vemurafenib Pracinostat 1.0000 0.1067
uM
 48
hr
 2 1651 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 118.63 NGFR rabbit IgG(H+L) / donkey NGFR 885.63
WM115-B2-rep2
F4
WM-115 Vemurafenib BIX-01294 1.0000 0.1067
uM
 48
hr
 2 1551 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 158.43 NGFR rabbit IgG(H+L) / donkey NGFR 1178.42
WM115-B2-rep2
F5
WM-115 Vemurafenib Methylstat 1.0000 0.1067
uM
 48
hr
 2 1428 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 125.16 NGFR rabbit IgG(H+L) / donkey NGFR 1139.78
WM115-B2-rep2
F6
WM-115 Vemurafenib I-BET151 1.0000 0.1067
uM
 48
hr
 2 1356 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 127.15 NGFR rabbit IgG(H+L) / donkey NGFR 855.29
WM115-B2-rep2
F7
WM-115 Vemurafenib CX-5461 1.0000 0.1067
uM
 48
hr
 2 1332 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 124.12 NGFR rabbit IgG(H+L) / donkey NGFR 1219.65
WM115-B2-rep2
F8
WM-115 Vemurafenib RG-108 1.0000 0.1067
uM
 48
hr
 2 1201 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 137.97 NGFR rabbit IgG(H+L) / donkey NGFR 1055.77
WM115-B2-rep2
G2
WM-115 Vemurafenib Anacardic acid 1.0000 0.1067
uM
 48
hr
 2 1318 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 127.59 NGFR rabbit IgG(H+L) / donkey NGFR 1134.58
WM115-B2-rep2
G3
WM-115 Vemurafenib MC1568 1.0000 0.1067
uM
 48
hr
 2 1314 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 153.34 NGFR rabbit IgG(H+L) / donkey NGFR 1125.66
WM115-B2-rep2
G4
WM-115 Vemurafenib UNC0638 1.0000 0.1067
uM
 48
hr
 2 1283 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 143.28 NGFR rabbit IgG(H+L) / donkey NGFR 1261.16
WM115-B2-rep2
G5
WM-115 Vemurafenib Tranylcypromine 1.0000 0.1067
uM
 48
hr
 2 1288 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 136.03 NGFR rabbit IgG(H+L) / donkey NGFR 1117.36
WM115-B2-rep2
G6
WM-115 Vemurafenib Ischemin 1.0000 0.1067
uM
 48
hr
 2 1341 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 120.71 NGFR rabbit IgG(H+L) / donkey NGFR 1230.55
WM115-B2-rep2
G7
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1150 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 140.75 NGFR rabbit IgG(H+L) / donkey NGFR 1097.89
WM115-B2-rep2
H10
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1002 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 123.83 NGFR rabbit IgG(H+L) / donkey NGFR 994.93
WM115-B2-rep2
H11
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1108 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 148.07 NGFR rabbit IgG(H+L) / donkey NGFR 1162.21
WM115-B2-rep2
H2
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1162 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 135.28 NGFR rabbit IgG(H+L) / donkey NGFR 1154.34
WM115-B2-rep2
H3
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1118 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 130.53 NGFR rabbit IgG(H+L) / donkey NGFR 1078.75
WM115-B2-rep2
H4
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1141 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 126.91 NGFR rabbit IgG(H+L) / donkey NGFR 1132.64
WM115-B2-rep2
H5
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1092 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 125.32 NGFR rabbit IgG(H+L) / donkey NGFR 1076.20
WM115-B2-rep2
H6
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1059 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 127.16 NGFR rabbit IgG(H+L) / donkey NGFR 1102.52
WM115-B2-rep2
H7
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1144 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 134.31 NGFR rabbit IgG(H+L) / donkey NGFR 1008.74
WM115-B2-rep2
H8
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1118 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 119.18 NGFR rabbit IgG(H+L) / donkey NGFR 1039.92
WM115-B2-rep2
H9