Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
WM-115 Vemurafenib Tranylcypromine 1.0000 0.1067
uM
 48
hr
 1 1145 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 125.44 NGFR rabbit IgG(H+L) / donkey NGFR 997.49
WM115-B2-rep1
G6
WM-115 Vemurafenib Ischemin 1.0000 0.1067
uM
 48
hr
 1 1092 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 140.85 NGFR rabbit IgG(H+L) / donkey NGFR 1107.34
WM115-B2-rep1
G7
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 883 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 154.08 NGFR rabbit IgG(H+L) / donkey NGFR 1102.94
WM115-B2-rep1
H10
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 952 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 139.09 NGFR rabbit IgG(H+L) / donkey NGFR 915.79
WM115-B2-rep1
H11
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 939 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 135.31 NGFR rabbit IgG(H+L) / donkey NGFR 946.36
WM115-B2-rep1
H2
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 845 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 143.87 NGFR rabbit IgG(H+L) / donkey NGFR 1057.39
WM115-B2-rep1
H3
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 808 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 136.62 NGFR rabbit IgG(H+L) / donkey NGFR 1052.15
WM115-B2-rep1
H4
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 746 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 129.54 NGFR rabbit IgG(H+L) / donkey NGFR 1007.03
WM115-B2-rep1
H5
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 904 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 125.03 NGFR rabbit IgG(H+L) / donkey NGFR 939.97
WM115-B2-rep1
H6
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 861 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 129.45 NGFR rabbit IgG(H+L) / donkey NGFR 1013.19
WM115-B2-rep1
H7
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 876 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 134.53 NGFR rabbit IgG(H+L) / donkey NGFR 1049.12
WM115-B2-rep1
H8
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 906 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 145.77 NGFR rabbit IgG(H+L) / donkey NGFR 1117.05
WM115-B2-rep1
H9
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1100 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 149.13 NGFR rabbit IgG(H+L) / donkey NGFR 1179.05
WM115-B2-rep2
A10
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1179 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 152.88 NGFR rabbit IgG(H+L) / donkey NGFR 1171.65
WM115-B2-rep2
A11
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1222 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 158.91 NGFR rabbit IgG(H+L) / donkey NGFR 1104.02
WM115-B2-rep2
A2
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1434 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 159.89 NGFR rabbit IgG(H+L) / donkey NGFR 1166.16
WM115-B2-rep2
A3
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1183 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 156.49 NGFR rabbit IgG(H+L) / donkey NGFR 1100.47
WM115-B2-rep2
A4
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1049 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 121.43 NGFR rabbit IgG(H+L) / donkey NGFR 1010.51
WM115-B2-rep2
A5
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1174 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 152.28 NGFR rabbit IgG(H+L) / donkey NGFR 1115.34
WM115-B2-rep2
A6
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1123 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 140.11 NGFR rabbit IgG(H+L) / donkey NGFR 1122.60
WM115-B2-rep2
A7
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 956 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 128.27 NGFR rabbit IgG(H+L) / donkey NGFR 1161.55
WM115-B2-rep2
A8
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1162 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 148.94 NGFR rabbit IgG(H+L) / donkey NGFR 1096.98
WM115-B2-rep2
A9
WM-115 Vemurafenib Olaparib 1.0000 0.1067
uM
 48
hr
 2 1291 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 154.34 NGFR rabbit IgG(H+L) / donkey NGFR 1162.67
WM115-B2-rep2
B2
WM-115 Vemurafenib Iniparib 1.0000 0.1067
uM
 48
hr
 2 1353 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 144.10 NGFR rabbit IgG(H+L) / donkey NGFR 1083.77
WM115-B2-rep2
B3
WM-115 Vemurafenib CTB 1.0000 0.1067
uM
 48
hr
 2 1217 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 137.89 NGFR rabbit IgG(H+L) / donkey NGFR 1057.42
WM115-B2-rep2
B4