Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib Palbociclib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 573.25 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 137.42 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 113.14
A375-P10
B11
A-375 Vemurafenib Palbociclib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 118.73 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 92.50 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 196.46
A375-P10
C11
A-375 Vemurafenib PF562271 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 475.12 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 150.33 NGFR rabbit IgG(H+L) / donkey NGFR 510.86
A375-P1
F9
A-375 Vemurafenib PF562271 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 506.69 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 149.46 NGFR rabbit IgG(H+L) / donkey NGFR 429.87
A375-P1
G9
A-375 Vemurafenib Saracatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2305.08 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 453.76 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 213.68
A375-P10
B3
A-375 Vemurafenib JNK-IN-8 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1645.39 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 328.55 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 123.98
A375-P10
B9
A-375 Vemurafenib Dasatinib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 523.10 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 171.20 NGFR rabbit IgG(H+L) / donkey NGFR 138.23
A375-P1
F11
A-375 Vemurafenib Defactinib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 492.21 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 138.59 NGFR rabbit IgG(H+L) / donkey NGFR 204.04
A375-P1
F7
A-375 Vemurafenib Defactinib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 344.82 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 136.28 NGFR rabbit IgG(H+L) / donkey NGFR 282.18
A375-P1
D7
A-375 Vemurafenib Defactinib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 576.29 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 147.69 NGFR rabbit IgG(H+L) / donkey NGFR 331.16
A375-P1
G7
A-375 Vemurafenib PF562271 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 523.94 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 163.91 NGFR rabbit IgG(H+L) / donkey NGFR 465.12
A375-P1
D9
A-375 Vemurafenib PF562271 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1095.66 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 214.29 NGFR rabbit IgG(H+L) / donkey NGFR 135.55
A375-P1
E9
A-375 Vemurafenib Dasatinib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1734.18 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 353.69 NGFR rabbit IgG(H+L) / donkey NGFR 119.95
A375-P1
E11
A-375 Vemurafenib Pictilisib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1326.42 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 315.74 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 174.73
A375-P10
B5
A-375 Vemurafenib Defactinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1985.46 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 359.03 NGFR rabbit IgG(H+L) / donkey NGFR 315.78
A375-P1
B7
A-375 Vemurafenib Defactinib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1610.17 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 333.93 NGFR rabbit IgG(H+L) / donkey NGFR 343.07
A375-P1
E7
A-375 Vemurafenib Defactinib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 476.63 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 143.31 NGFR rabbit IgG(H+L) / donkey NGFR 248.80
A375-P1
C7
A-375 Vemurafenib Dasatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2000.92 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 389.89 NGFR rabbit IgG(H+L) / donkey NGFR 116.46
A375-P1
B11
A-375 Vemurafenib PF562271 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 516.73 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 165.19 NGFR rabbit IgG(H+L) / donkey NGFR 493.56
A375-P1
C9
A-375 Vemurafenib PF562271 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1339.47 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 253.18 NGFR rabbit IgG(H+L) / donkey NGFR 154.21
A375-P1
B9
A-375 Vemurafenib Dasatinib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 540.13 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 175.10 NGFR rabbit IgG(H+L) / donkey NGFR 180.58
A375-P1
D11
A-375 Vemurafenib Dasatinib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 471.76 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 163.07 NGFR rabbit IgG(H+L) / donkey NGFR 153.81
A375-P1
G11
A-375 Vemurafenib Dasatinib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 559.44 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 178.24 NGFR rabbit IgG(H+L) / donkey NGFR 158.24
A375-P1
C11
A-375 Vemurafenib Pictilisib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 542.42 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 186.55 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 217.56
A375-P10
C5
A-375 Vemurafenib Saracatinib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 465.64 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 166.19 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 247.07
A375-P10
C3
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  • 1 to 25 of 1440 records