Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib PF562271 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1095.66 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 214.29 NGFR rabbit IgG(H+L) / donkey NGFR 135.55
A375-P1
E9
A-375 Vemurafenib Saracatinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2084.99 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 403.10 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 190.22
A375-P10
B2
A-375 Vemurafenib Trametinib 0.000 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 445.42 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 156.24 NGFR rabbit IgG(H+L) / donkey NGFR 1195.67
A375-P1
E5
A-375 Vemurafenib PF562271 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2421.26 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 433.32 NGFR rabbit IgG(H+L) / donkey NGFR 252.56
A375-P1
E8
A-375 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3098.11 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 540.77 NGFR rabbit IgG(H+L) / donkey NGFR 567.23
A375-P1
E2
A-375 Vemurafenib Palbociclib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 573.25 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 137.42 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 113.14
A375-P10
B11
A-375 Vemurafenib Dasatinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2722.08 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 510.86 NGFR rabbit IgG(H+L) / donkey NGFR 141.68
A375-P1
B10
A-375 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 539.06 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 158.41 NGFR rabbit IgG(H+L) / donkey NGFR 1132.49
A375-P1
E4
A-375 Vemurafenib Dasatinib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2728.23 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 495.24 NGFR rabbit IgG(H+L) / donkey NGFR 132.92
A375-P1
E10
A-375 Vemurafenib Defactinib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1610.17 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 333.93 NGFR rabbit IgG(H+L) / donkey NGFR 343.07
A375-P1
E7
A-375 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3238.32 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 574.69 NGFR rabbit IgG(H+L) / donkey NGFR 547.66
A375-P1
E3
A-375 Vemurafenib 0.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3094.28 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 552.89 NGFR rabbit IgG(H+L) / donkey NGFR 593.97
A375-P1
B2
A-375 Vemurafenib Defactinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2523.19 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 469.18 NGFR rabbit IgG(H+L) / donkey NGFR 305.98
A375-P1
B6
A-375 Vemurafenib Palbociclib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 745.75 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 167.99 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 134.37
A375-P10
B10
A-375 Vemurafenib Defactinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1985.46 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 359.03 NGFR rabbit IgG(H+L) / donkey NGFR 315.78
A375-P1
B7
A-375 Vemurafenib 0.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2981.15 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 513.45 NGFR rabbit IgG(H+L) / donkey NGFR 573.44
A375-P1
B3
A-375 Vemurafenib PF562271 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2497.37 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 448.69 NGFR rabbit IgG(H+L) / donkey NGFR 257.39
A375-P1
B8
A-375 Vemurafenib Dasatinib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1734.18 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 353.69 NGFR rabbit IgG(H+L) / donkey NGFR 119.95
A375-P1
E11
A-375 Vemurafenib PF562271 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1339.47 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 253.18 NGFR rabbit IgG(H+L) / donkey NGFR 154.21
A375-P1
B9
A-375 Vemurafenib Dasatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2000.92 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 389.89 NGFR rabbit IgG(H+L) / donkey NGFR 116.46
A375-P1
B11
A-375 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 521.55 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 174.42 NGFR rabbit IgG(H+L) / donkey NGFR 1215.18
A375-P1
B4
A-375 Vemurafenib Defactinib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2351.55 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 431.86 NGFR rabbit IgG(H+L) / donkey NGFR 295.59
A375-P1
E6
A-375 Vemurafenib Trametinib 0.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 496.87 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 163.74 NGFR rabbit IgG(H+L) / donkey NGFR 1256.19
A375-P1
B5
A-375 Vemurafenib Pictilisib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1919.86 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 391.41 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 234.89
A375-P10
B4
A-375 Vemurafenib Saracatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2305.08 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 453.76 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 213.68
A375-P10
B3
  • Page 1 of 58
  • 1 to 25 of 1440 records