Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 316.96 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 141.63 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 839.25
A375-P3
B4
A-375 Vemurafenib Trametinib 0.316 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 313.31 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 129.41 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 713.04
A375-P3
F4
A-375 Vemurafenib Trametinib 1.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 434.07 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 144.84 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1630.59
A375-P2
D4
A-375 Vemurafenib Trametinib 1.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 438.34 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 143.63 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1658.90
A375-P2
G4
A-375 Vemurafenib Trametinib 1.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 346.07 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 142.60 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 856.27
A375-P3
D4
A-375 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 388.49 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 147.04 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 863.20
A375-P3
E4
A-375 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 466.25 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 160.61 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1844.28
A375-P2
B4
A-375 Vemurafenib Trametinib 0.316 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 421.67 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 150.08 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1700.88
A375-P2
C4
A-375 Vemurafenib AZD8055 1.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 534.00 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 177.96 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 299.97
A375-P10
D6
A-375 Vemurafenib Trametinib 0.316 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 464.67 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 146.28 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1579.99
A375-P2
F4
A-375 Vemurafenib Trametinib 0.316 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 302.60 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 140.61 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 828.66
A375-P3
C4
A-375 Vemurafenib AZD8055 0.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1301.07 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 298.21 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 167.81
A375-P10
E6
A-375 Vemurafenib AZD8055 0.316 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 774.02 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 199.06 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 258.33
A375-P10
F6
A-375 Vemurafenib Trametinib 0.316 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 475.69 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 152.94 NGFR rabbit IgG(H+L) / donkey NGFR 1151.49
A375-P1
C4
A-375 Vemurafenib Trametinib 1.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 550.54 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 169.58 NGFR rabbit IgG(H+L) / donkey NGFR 1008.81
A375-P1
D4
A-375 Vemurafenib AZD8055 1.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 809.04 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 194.24 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 290.26
A375-P10
G6
A-375 Vemurafenib Trametinib 1.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 519.89 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 151.01 NGFR rabbit IgG(H+L) / donkey NGFR 890.02
A375-P1
G4
A-375 Vemurafenib AZD8055 0.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1461.59 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 333.92 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 205.97
A375-P10
B6
A-375 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 521.55 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 174.42 NGFR rabbit IgG(H+L) / donkey NGFR 1215.18
A375-P1
B4
A-375 Vemurafenib AZD8055 0.316 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 892.27 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 227.01 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 314.05
A375-P10
C6
A-375 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 436.86 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 144.14 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1582.79
A375-P2
E4
A-375 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 539.06 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 158.41 NGFR rabbit IgG(H+L) / donkey NGFR 1132.49
A375-P1
E4
A-375 Vemurafenib Trametinib 0.316 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 517.71 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 155.94 NGFR rabbit IgG(H+L) / donkey NGFR 1172.91
A375-P1
F4
A-375 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 230.23 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 126.27 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 293.14
A375-P4
B4
A-375 Vemurafenib Trametinib 1.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 367.34 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 145.41 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 738.84
A375-P3
G4
  • Page 1 of 58
  • 1 to 25 of 1440 records