Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 373.0 7
0088-0195
686.08 194.73 240 57.94 0.898
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 289.3 7
0089-0197
218.70 197.96 244 59.36 0.870
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 324.8 7
0090-0198
314.70 198.25 251 58.77 0.913
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 473.5 7
0091-0196
613.26 196.59 204 56.87 0.793
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 302.3 7
0092-0201
946.42 201.19 274 66.08 0.788
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 303.8 7
0093-0206
58.01 206.48 258 61.36 0.861
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 313.0 7
0094-0211
177.98 211.36 360 69.60 0.934
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 440.1 7
0095-0216
397.84 216.51 337 69.26 0.883
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 250.9 7
0096-0218
339.99 218.35 273 60.77 0.929
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 463.8 7
0097-0221
569.60 222.01 391 87.50 0.642
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 293.2 7
0098-0219
89.92 218.98 242 57.11 0.932
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 308.0 7
0099-0225
446.62 225.98 396 76.43 0.852
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 315.4 7
0100-0228
126.72 228.49 240 56.77 0.936
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 418.8 7
0101-0228
413.78 228.49 276 61.36 0.921
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 247.4 7
0102-0231
907.18 231.20 289 63.36 0.905
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 632.8 7
0103-0232
720.98 231.94 236 62.67 0.755
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 633.5 7
0104-0233
264.91 234.22 194 56.53 0.763
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 474.7 7
0105-0234
283.80 234.69 332 68.43 0.891
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 329.4 7
0106-0237
521.36 236.40 382 73.01 0.900
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 372.3 7
0107-0236
947.56 236.18 340 68.77 0.903
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 312.0 7
0108-0237
872.17 237.48 266 63.84 0.820
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 13588.8 7
0109-0245
843.77 243.71 350 102.57 0.418
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 484.2 7
0110-0243
51.98 243.74 182 49.70 0.926
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 352.0 7
0111-0243
604.19 243.46 216 59.36 0.770
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 339.4 7
0112-0246
378.93 246.46 256 59.36 0.913