COLO 858 - Cell Line (ID:50149)

HMS LINCS ID: 50149
Name: COLO 858
Alternative Names:
LINCS ID: LCL-1242
Alternative ID: CLO_0002569
Parent Cell Line:
Reference Source: European Collection of Authenticated Cell Cultures (ECACC) 93052613
Organism: Homo sapiens
Organ: skin
Tissue:
Cell Type:
Details of Cell Type:
Donor Sex: male
Donor Age:
Donor Ethnicity:
Donor Health Status:
Disease: DOID:1909, malignant melanoma
Details of Disease:
Production Details:
Genetic Modification(s): none
Known Mutations:
Citation Information for Mutations:
Verification Reference Profile:
Growth Properties: adherent
Recommended Culture Conditions: From MGH/CMT as specified by cell provider: RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 37C; 5% CO2.
Relevant Citations:
Usage Note:
Comments:
Date Publicly Available: 2012-07-31
Most Recent Update: 2016-08-24

Batch Information for HMSL50149-3:

HMS LINCS Batch ID: 50149-3
Provider: Mohammad Fallahi-Sichani (Harvard Medical School)
Provider Catalog ID:
Provider Batch ID:
Source Information: Obtained by Mohammad Fallahi-Sichani (Harvard Medical School) from Nathan Moerke (Harvard Medical School) and distributed to Jia-Ren Lin in October, 2014. Moerke previously had obtained the cells from the Center for Molecular Therapeutics (CMT; Massachusetts General Hospital) (contact: Cyril Benes).
Date Received: 2012-10-29
HMS QC Outcome:
Transient Modification(s):
Comments:
Date Publicly Available: 2016-06-06
Most Recent Update: 2016-07-12