H3 (pS10) - Antibody (ID:80001)

HMS LINCS ID: 80001
Name: H3 (pS10)
Alternative Names: Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) #3475
Clone Name: D2C8
Resource Identification Initiative ID: AB_10694639
LINCS ID:
Type: natural
Nominal Protein Target(s): H3 (pS10)
Nominal Protein Target HMS LINCS ID(s): 201294
Nominal Non-protein Target:
Organism of Nominal Target: Homo sapiens
Other Target Information: Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) detects endogenous levels of histone H3 only when phosphorylated at Ser10. The antibody does not cross-react with other phosphorylated histones or with acetylated histones. Species Reactivity: Human, Mouse, Rat, Monkey, Zebrafish. (source: Cell Signaling Technology)
Other Human Protein Target HMS LINCS ID(s):
Immunogen: Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser10 of human histone H3. (source: Cell Signaling Technology)
Immunogen Sequence:
Fc Domain Source Species: rabbit
Clonality: monoclonal
Fc Domain Isotype: IgG
Source Organism for Production: rabbit
Production Details:
Conjugate or Label: Alexa Fluor 555
Labeling Details: This antibody was conjugated to Alexa Fluor® 555 under optimal conditions with an F/P ratio of 2-6. (source: Cell Signaling Technology)
Relevant Citations:
Date Publicly Available: 2015-09-16
Most Recent Update: 2016-09-14

Datasets:

HMS Dataset ID Dataset Title HMS Dataset Type
20236
Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line
Microscopy/Imaging
20267
Highly-multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the MCF 10A breast cell line treated with EGF or four kinase inhibitors
Microscopy/Imaging
20303
LINCS MCF 10A Common Project: Highly-multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the MCF 10A breast cell line treated with 8 small molecule perturbagens. Dataset 1 of 6: Cytosolic fluorescent intensity measurements at 24 hr.
Microscopy/Imaging
20304
LINCS MCF 10A Common Project: Highly-multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the MCF 10A breast cell line treated with 8 small molecule perturbagens. Dataset 2 of 6: Nuclear fluorescent intensity measurements at 24 hr.
Microscopy/Imaging
20305
LINCS MCF 10A Common Project: Highly-multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the MCF 10A breast cell line treated with 8 small molecule perturbagens. Dataset 3 of 6: Cytosolic fluorescent intensity measurements at 48 hr.
Microscopy/Imaging
20306
LINCS MCF 10A Common Project: Highly-multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the MCF 10A breast cell line treated with 8 small molecule perturbagens. Dataset 4 of 6: Nuclear fluorescent intensity measurements at 48 hr.
Microscopy/Imaging
20307
LINCS MCF 10A Common Project: Highly-multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the MCF 10A breast cell line treated with 8 small molecule perturbagens. Dataset 5 of 6: Cytosolic fluorescent intensity measurements at 72 hr.
Microscopy/Imaging
20308
LINCS MCF 10A Common Project: Highly-multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the MCF 10A breast cell line treated with 8 small molecule perturbagens. Dataset 6 of 6: Nuclear fluorescent intensity measurements at 72 hr.
Microscopy/Imaging

Batch Information:

HMS LINCS Batch ID Provider Provider Catalog ID Provider Batch ID
80001-1 Cell Signaling Technology 3475S 2