Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
WM-115 Vemurafenib Olaparib 1.0000 0.5333
uM
 48
hr
 2 1251 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 146.30 NGFR rabbit IgG(H+L) / donkey NGFR 1097.07
WM115-B1-rep2
B2
WM-115 Vemurafenib Iniparib 1.0000 0.5333
uM
 48
hr
 2 1282 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 138.32 NGFR rabbit IgG(H+L) / donkey NGFR 1147.41
WM115-B1-rep2
B3
WM-115 Vemurafenib CTB 1.0000 0.5333
uM
 48
hr
 2 1386 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 141.51 NGFR rabbit IgG(H+L) / donkey NGFR 1016.82
WM115-B1-rep2
B4
WM-115 Vemurafenib Rocilinostat 1.0000 0.5333
uM
 48
hr
 2 1233 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 128.64 NGFR rabbit IgG(H+L) / donkey NGFR 894.74
WM115-B1-rep2
B5
WM-115 Vemurafenib GSK-J1 1.0000 0.5333
uM
 48
hr
 2 1323 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 139.46 NGFR rabbit IgG(H+L) / donkey NGFR 1138.62
WM115-B1-rep2
B6
WM-115 Vemurafenib PFI-1 1.0000 0.5333
uM
 48
hr
 2 1302 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 145.78 NGFR rabbit IgG(H+L) / donkey NGFR 1035.27
WM115-B1-rep2
B7
WM-115 Vemurafenib UNC669 1.0000 0.5333
uM
 48
hr
 2 1455 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 141.47 NGFR rabbit IgG(H+L) / donkey NGFR 1276.18
WM115-B1-rep2
B8
WM-115 Vemurafenib Veliparib 1.0000 0.5333
uM
 48
hr
 2 1316 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 151.72 NGFR rabbit IgG(H+L) / donkey NGFR 1100.71
WM115-B1-rep2
C2
WM-115 Vemurafenib Rucaparib 1.0000 0.5333
uM
 48
hr
 2 1408 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 147.49 NGFR rabbit IgG(H+L) / donkey NGFR 1104.73
WM115-B1-rep2
C3
WM-115 Vemurafenib Belinostat 1.0000 0.5333
uM
 48
hr
 2 1206 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 142.06 NGFR rabbit IgG(H+L) / donkey NGFR 358.18
WM115-B1-rep2
C4
WM-115 Vemurafenib Selisistat 1.0000 0.5333
uM
 48
hr
 2 1484 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 134.67 NGFR rabbit IgG(H+L) / donkey NGFR 1061.55
WM115-B1-rep2
C5
WM-115 Vemurafenib GSK-J2 1.0000 0.5333
uM
 48
hr
 2 1550 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 149.34 NGFR rabbit IgG(H+L) / donkey NGFR 1141.48
WM115-B1-rep2
C6
WM-115 Vemurafenib (+)-JQ1 1.0000 0.5333
uM
 48
hr
 2 1082 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 123.57 NGFR rabbit IgG(H+L) / donkey NGFR 548.46
WM115-B1-rep2
C7
WM-115 Vemurafenib UNC1215 1.0000 0.5333
uM
 48
hr
 2 1477 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 142.54 NGFR rabbit IgG(H+L) / donkey NGFR 1208.27
WM115-B1-rep2
C8
WM-115 Vemurafenib Vorinostat 1.0000 0.5333
uM
 48
hr
 2 1514 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 135.65 NGFR rabbit IgG(H+L) / donkey NGFR 957.47
WM115-B1-rep2
D2
WM-115 Vemurafenib JW55 1.0000 0.5333
uM
 48
hr
 2 1270 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 138.41 NGFR rabbit IgG(H+L) / donkey NGFR 1093.58
WM115-B1-rep2
D3
WM-115 Vemurafenib Entinostat 1.0000 0.5333
uM
 48
hr
 2 1744 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 121.89 NGFR rabbit IgG(H+L) / donkey NGFR 1128.25
WM115-B1-rep2
D4
WM-115 Vemurafenib AGK2 1.0000 0.5333
uM
 48
hr
 2 1023 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 128.12 NGFR rabbit IgG(H+L) / donkey NGFR 1175.15
WM115-B1-rep2
D5
WM-115 Vemurafenib GSK-J4 1.0000 0.5333
uM
 48
hr
 2 1614 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 137.78 NGFR rabbit IgG(H+L) / donkey NGFR 1200.46
WM115-B1-rep2
D6
WM-115 Vemurafenib (-)-JQ1 1.0000 0.5333
uM
 48
hr
 2 1595 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 134.52 NGFR rabbit IgG(H+L) / donkey NGFR 1222.46
WM115-B1-rep2
D7
WM-115 Vemurafenib IOX2 1.0000 0.5333
uM
 48
hr
 2 1361 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 124.11 NGFR rabbit IgG(H+L) / donkey NGFR 1071.77
WM115-B1-rep2
D8
WM-115 Vemurafenib Azacitidine 1.0000 0.5333
uM
 48
hr
 2 1168 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 159.86 NGFR rabbit IgG(H+L) / donkey NGFR 911.40
WM115-B1-rep2
E2
WM-115 Vemurafenib C646 1.0000 0.5333
uM
 48
hr
 2 1128 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 128.60 NGFR rabbit IgG(H+L) / donkey NGFR 1048.49
WM115-B1-rep2
E3
WM-115 Vemurafenib Mocetinostat 1.0000 0.5333
uM
 48
hr
 2 1590 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 119.27 NGFR rabbit IgG(H+L) / donkey NGFR 1037.35
WM115-B1-rep2
E4
WM-115 Vemurafenib Resveratrol 1.0000 0.5333
uM
 48
hr
 2 1298 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 140.55 NGFR rabbit IgG(H+L) / donkey NGFR 1087.58
WM115-B1-rep2
E5