Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
WM-115 Vemurafenib Daminozide 1.0000 0.5333
uM
 48
hr
 2 1419 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 134.10 NGFR rabbit IgG(H+L) / donkey NGFR 1163.35
WM115-B1-rep2
E6
WM-115 Vemurafenib I-BET 1.0000 0.5333
uM
 48
hr
 2 1311 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 122.35 NGFR rabbit IgG(H+L) / donkey NGFR 741.26
WM115-B1-rep2
E7
WM-115 Vemurafenib Epigallocatechin gallate 1.0000 0.5333
uM
 48
hr
 2 1274 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 125.39 NGFR rabbit IgG(H+L) / donkey NGFR 1161.43
WM115-B1-rep2
E8
WM-115 Vemurafenib Decitabine 1.0000 0.5333
uM
 48
hr
 2 1233 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 145.00 NGFR rabbit IgG(H+L) / donkey NGFR 918.47
WM115-B1-rep2
F2
WM-115 Vemurafenib Garcinol 1.0000 0.5333
uM
 48
hr
 2 1114 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 122.06 NGFR rabbit IgG(H+L) / donkey NGFR 1016.89
WM115-B1-rep2
F3
WM-115 Vemurafenib Pracinostat 1.0000 0.5333
uM
 48
hr
 2 1417 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 129.20 NGFR rabbit IgG(H+L) / donkey NGFR 609.07
WM115-B1-rep2
F4
WM-115 Vemurafenib BIX-01294 1.0000 0.5333
uM
 48
hr
 2 1271 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 175.73 NGFR rabbit IgG(H+L) / donkey NGFR 947.40
WM115-B1-rep2
F5
WM-115 Vemurafenib Methylstat 1.0000 0.5333
uM
 48
hr
 2 1188 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 118.50 NGFR rabbit IgG(H+L) / donkey NGFR 1096.56
WM115-B1-rep2
F6
WM-115 Vemurafenib I-BET151 1.0000 0.5333
uM
 48
hr
 2 1142 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 125.08 NGFR rabbit IgG(H+L) / donkey NGFR 686.83
WM115-B1-rep2
F7
WM-115 Vemurafenib CX-5461 1.0000 0.5333
uM
 48
hr
 2 1172 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 115.71 NGFR rabbit IgG(H+L) / donkey NGFR 1084.50
WM115-B1-rep2
F8
WM-115 Vemurafenib RG-108 1.0000 0.5333
uM
 48
hr
 2 1105 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 134.17 NGFR rabbit IgG(H+L) / donkey NGFR 1056.85
WM115-B1-rep2
G2
WM-115 Vemurafenib Anacardic acid 1.0000 0.5333
uM
 48
hr
 2 860 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 130.06 NGFR rabbit IgG(H+L) / donkey NGFR 1034.36
WM115-B1-rep2
G3
WM-115 Vemurafenib MC1568 1.0000 0.5333
uM
 48
hr
 2 996 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 169.70 NGFR rabbit IgG(H+L) / donkey NGFR 1014.41
WM115-B1-rep2
G4
WM-115 Vemurafenib UNC0638 1.0000 0.5333
uM
 48
hr
 2 1061 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 135.43 NGFR rabbit IgG(H+L) / donkey NGFR 1105.49
WM115-B1-rep2
G5
WM-115 Vemurafenib Tranylcypromine 1.0000 0.5333
uM
 48
hr
 2 900 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 138.52 NGFR rabbit IgG(H+L) / donkey NGFR 1071.63
WM115-B1-rep2
G6
WM-115 Vemurafenib Ischemin 1.0000 0.5333
uM
 48
hr
 2 1053 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 118.90 NGFR rabbit IgG(H+L) / donkey NGFR 1123.26
WM115-B1-rep2
G7
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 892 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 125.44 NGFR rabbit IgG(H+L) / donkey NGFR 1032.39
WM115-B1-rep2
H10
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 888 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 131.88 NGFR rabbit IgG(H+L) / donkey NGFR 1119.81
WM115-B1-rep2
H11
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 815 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 137.73 NGFR rabbit IgG(H+L) / donkey NGFR 976.81
WM115-B1-rep2
H2
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 815 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 132.56 NGFR rabbit IgG(H+L) / donkey NGFR 951.83
WM115-B1-rep2
H3
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 779 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 130.47 NGFR rabbit IgG(H+L) / donkey NGFR 983.28
WM115-B1-rep2
H4
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 841 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 142.81 NGFR rabbit IgG(H+L) / donkey NGFR 1076.91
WM115-B1-rep2
H5
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 821 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 124.47 NGFR rabbit IgG(H+L) / donkey NGFR 902.73
WM115-B1-rep2
H6
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 747 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 127.67 NGFR rabbit IgG(H+L) / donkey NGFR 1024.66
WM115-B1-rep2
H7
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 895 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 133.80 NGFR rabbit IgG(H+L) / donkey NGFR 971.63
WM115-B1-rep2
H8