Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib Pracinostat 0.3160 0.1067
uM
 48
hr
 1 912 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 219.92 NGFR rabbit IgG(H+L) / donkey NGFR 150.93
Colo858-B2-rep1
F4
COLO 858 Vemurafenib BIX-01294 0.3160 0.1067
uM
 48
hr
 1 2175 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 374.62 NGFR rabbit IgG(H+L) / donkey NGFR 150.11
Colo858-B2-rep1
F5
COLO 858 Vemurafenib Methylstat 0.3160 0.1067
uM
 48
hr
 1 2248 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 279.63 NGFR rabbit IgG(H+L) / donkey NGFR 146.80
Colo858-B2-rep1
F6
COLO 858 Vemurafenib I-BET151 0.3160 0.1067
uM
 48
hr
 1 1645 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 181.50 NGFR rabbit IgG(H+L) / donkey NGFR 33.08
Colo858-B2-rep1
F7
COLO 858 Vemurafenib CX-5461 0.3160 0.1067
uM
 48
hr
 1 2007 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 202.42 NGFR rabbit IgG(H+L) / donkey NGFR 240.61
Colo858-B2-rep1
F8
COLO 858 Vemurafenib RG-108 0.3160 0.1067
uM
 48
hr
 1 1249 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 276.06 NGFR rabbit IgG(H+L) / donkey NGFR 152.83
Colo858-B2-rep1
G2
COLO 858 Vemurafenib Anacardic acid 0.3160 0.1067
uM
 48
hr
 1 1144 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 317.68 NGFR rabbit IgG(H+L) / donkey NGFR 153.35
Colo858-B2-rep1
G3
COLO 858 Vemurafenib MC1568 0.3160 0.1067
uM
 48
hr
 1 1269 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 281.92 NGFR rabbit IgG(H+L) / donkey NGFR 115.06
Colo858-B2-rep1
G4
COLO 858 Vemurafenib UNC0638 0.3160 0.1067
uM
 48
hr
 1 1331 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 310.02 NGFR rabbit IgG(H+L) / donkey NGFR 183.08
Colo858-B2-rep1
G5
COLO 858 Vemurafenib Tranylcypromine 0.3160 0.1067
uM
 48
hr
 1 1311 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 241.69 NGFR rabbit IgG(H+L) / donkey NGFR 141.30
Colo858-B2-rep1
G6
COLO 858 Vemurafenib Ischemin 0.3160 0.1067
uM
 48
hr
 1 1417 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 326.68 NGFR rabbit IgG(H+L) / donkey NGFR 144.77
Colo858-B2-rep1
G7
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1020 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 286.71 NGFR rabbit IgG(H+L) / donkey NGFR 114.57
Colo858-B2-rep1
H10
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 880 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 236.58 NGFR rabbit IgG(H+L) / donkey NGFR 135.95
Colo858-B2-rep1
H11
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 930 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 263.08 NGFR rabbit IgG(H+L) / donkey NGFR 134.83
Colo858-B2-rep1
H2
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 709 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 278.92 NGFR rabbit IgG(H+L) / donkey NGFR 116.26
Colo858-B2-rep1
H3
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 748 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 229.27 NGFR rabbit IgG(H+L) / donkey NGFR 160.07
Colo858-B2-rep1
H4
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 821 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 254.01 NGFR rabbit IgG(H+L) / donkey NGFR 137.66
Colo858-B2-rep1
H5
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 976 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 256.15 NGFR rabbit IgG(H+L) / donkey NGFR 124.71
Colo858-B2-rep1
H6
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 912 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 230.07 NGFR rabbit IgG(H+L) / donkey NGFR 122.34
Colo858-B2-rep1
H7
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 983 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 275.00 NGFR rabbit IgG(H+L) / donkey NGFR 146.13
Colo858-B2-rep1
H8
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 949 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 297.75 NGFR rabbit IgG(H+L) / donkey NGFR 140.37
Colo858-B2-rep1
H9
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 1485 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 347.49 NGFR rabbit IgG(H+L) / donkey NGFR 160.81
Colo858-B2-rep2
A10
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 1440 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 346.44 NGFR rabbit IgG(H+L) / donkey NGFR 179.05
Colo858-B2-rep2
A11
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 1358 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 340.31 NGFR rabbit IgG(H+L) / donkey NGFR 143.67
Colo858-B2-rep2
A2
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 1070 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 412.54 NGFR rabbit IgG(H+L) / donkey NGFR 139.52
Colo858-B2-rep2
A3