Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib PFI-1 0.3160 0.1067
uM
 48
hr
 1 1597 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 305.58 NGFR rabbit IgG(H+L) / donkey NGFR 152.98
Colo858-B2-rep1
B7
COLO 858 Vemurafenib UNC669 0.3160 0.1067
uM
 48
hr
 1 1631 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 318.51 NGFR rabbit IgG(H+L) / donkey NGFR 162.38
Colo858-B2-rep1
B8
COLO 858 Vemurafenib Veliparib 0.3160 0.1067
uM
 48
hr
 1 1792 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 277.98 NGFR rabbit IgG(H+L) / donkey NGFR 153.52
Colo858-B2-rep1
C2
COLO 858 Vemurafenib Rucaparib 0.3160 0.1067
uM
 48
hr
 1 2092 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 264.74 NGFR rabbit IgG(H+L) / donkey NGFR 163.85
Colo858-B2-rep1
C3
COLO 858 Vemurafenib Belinostat 0.3160 0.1067
uM
 48
hr
 1 798 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 187.52 NGFR rabbit IgG(H+L) / donkey NGFR 66.34
Colo858-B2-rep1
C4
COLO 858 Vemurafenib Selisistat 0.3160 0.1067
uM
 48
hr
 1 2270 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 335.67 NGFR rabbit IgG(H+L) / donkey NGFR 136.25
Colo858-B2-rep1
C5
COLO 858 Vemurafenib GSK-J2 0.3160 0.1067
uM
 48
hr
 1 2462 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 317.12 NGFR rabbit IgG(H+L) / donkey NGFR 150.12
Colo858-B2-rep1
C6
COLO 858 Vemurafenib (+)-JQ1 0.3160 0.1067
uM
 48
hr
 1 1564 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 110.51 NGFR rabbit IgG(H+L) / donkey NGFR 23.82
Colo858-B2-rep1
C7
COLO 858 Vemurafenib UNC1215 0.3160 0.1067
uM
 48
hr
 1 2333 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 313.71 NGFR rabbit IgG(H+L) / donkey NGFR 176.41
Colo858-B2-rep1
C8
COLO 858 Vemurafenib Vorinostat 0.3160 0.1067
uM
 48
hr
 1 1749 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 253.38 NGFR rabbit IgG(H+L) / donkey NGFR 166.28
Colo858-B2-rep1
D2
COLO 858 Vemurafenib JW55 0.3160 0.1067
uM
 48
hr
 1 2407 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 280.93 NGFR rabbit IgG(H+L) / donkey NGFR 154.25
Colo858-B2-rep1
D3
COLO 858 Vemurafenib Entinostat 0.3160 0.1067
uM
 48
hr
 1 3047 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 285.27 NGFR rabbit IgG(H+L) / donkey NGFR 129.34
Colo858-B2-rep1
D4
COLO 858 Vemurafenib AGK2 0.3160 0.1067
uM
 48
hr
 1 2399 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 279.22 NGFR rabbit IgG(H+L) / donkey NGFR 124.75
Colo858-B2-rep1
D5
COLO 858 Vemurafenib GSK-J4 0.3160 0.1067
uM
 48
hr
 1 3271 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 334.17 NGFR rabbit IgG(H+L) / donkey NGFR 153.69
Colo858-B2-rep1
D6
COLO 858 Vemurafenib (-)-JQ1 0.3160 0.1067
uM
 48
hr
 1 3394 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 313.83 NGFR rabbit IgG(H+L) / donkey NGFR 168.87
Colo858-B2-rep1
D7
COLO 858 Vemurafenib IOX2 0.3160 0.1067
uM
 48
hr
 1 3247 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 296.22 NGFR rabbit IgG(H+L) / donkey NGFR 170.23
Colo858-B2-rep1
D8
COLO 858 Vemurafenib Azacitidine 0.3160 0.1067
uM
 48
hr
 1 1758 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 254.56 NGFR rabbit IgG(H+L) / donkey NGFR 128.93
Colo858-B2-rep1
E2
COLO 858 Vemurafenib C646 0.3160 0.1067
uM
 48
hr
 1 2247 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 313.23 NGFR rabbit IgG(H+L) / donkey NGFR 149.21
Colo858-B2-rep1
E3
COLO 858 Vemurafenib Mocetinostat 0.3160 0.1067
uM
 48
hr
 1 2689 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 206.37 NGFR rabbit IgG(H+L) / donkey NGFR 98.91
Colo858-B2-rep1
E4
COLO 858 Vemurafenib Resveratrol 0.3160 0.1067
uM
 48
hr
 1 2616 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 310.59 NGFR rabbit IgG(H+L) / donkey NGFR 156.15
Colo858-B2-rep1
E5
COLO 858 Vemurafenib Daminozide 0.3160 0.1067
uM
 48
hr
 1 3016 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 329.69 NGFR rabbit IgG(H+L) / donkey NGFR 141.87
Colo858-B2-rep1
E6
COLO 858 Vemurafenib I-BET 0.3160 0.1067
uM
 48
hr
 1 2640 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 228.39 NGFR rabbit IgG(H+L) / donkey NGFR 75.66
Colo858-B2-rep1
E7
COLO 858 Vemurafenib Epigallocatechin gallate 0.3160 0.1067
uM
 48
hr
 1 2911 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 352.95 NGFR rabbit IgG(H+L) / donkey NGFR 169.38
Colo858-B2-rep1
E8
COLO 858 Vemurafenib Decitabine 0.3160 0.1067
uM
 48
hr
 1 1643 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 245.77 NGFR rabbit IgG(H+L) / donkey NGFR 163.41
Colo858-B2-rep1
F2
COLO 858 Vemurafenib Garcinol 0.3160 0.1067
uM
 48
hr
 1 1987 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 314.10 NGFR rabbit IgG(H+L) / donkey NGFR 143.60
Colo858-B2-rep1
F3