Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1371 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 358.01 NGFR rabbit IgG(H+L) / donkey NGFR 148.58
Colo858-A2-rep1
A9
COLO 858 Vemurafenib Olaparib 0.3160 2.6667
uM
 48
hr
 1 1345 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 290.82 NGFR rabbit IgG(H+L) / donkey NGFR 146.21
Colo858-A2-rep1
B2
COLO 858 Vemurafenib Iniparib 0.3160 2.6667
uM
 48
hr
 1 1703 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 310.30 NGFR rabbit IgG(H+L) / donkey NGFR 122.97
Colo858-A2-rep1
B3
COLO 858 Vemurafenib CTB 0.3160 2.6667
uM
 48
hr
 1 1748 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 291.14 NGFR rabbit IgG(H+L) / donkey NGFR 149.16
Colo858-A2-rep1
B4
COLO 858 Vemurafenib Rocilinostat 0.3160 2.6667
uM
 48
hr
 1 755 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 163.81 NGFR rabbit IgG(H+L) / donkey NGFR 184.22
Colo858-A2-rep1
B5
COLO 858 Vemurafenib GSK-J1 0.3160 2.6667
uM
 48
hr
 1 1988 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 344.25 NGFR rabbit IgG(H+L) / donkey NGFR 140.14
Colo858-A2-rep1
B6
COLO 858 Vemurafenib PFI-1 0.3160 2.6667
uM
 48
hr
 1 1383 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 138.34 NGFR rabbit IgG(H+L) / donkey NGFR 30.41
Colo858-A2-rep1
B7
COLO 858 Vemurafenib UNC669 0.3160 2.6667
uM
 48
hr
 1 2097 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 357.07 NGFR rabbit IgG(H+L) / donkey NGFR 157.23
Colo858-A2-rep1
B8
COLO 858 Vemurafenib Veliparib 0.3160 2.6667
uM
 48
hr
 1 2017 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 297.02 NGFR rabbit IgG(H+L) / donkey NGFR 148.72
Colo858-A2-rep1
C2
COLO 858 Vemurafenib Rucaparib 0.3160 2.6667
uM
 48
hr
 1 2594 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 255.48 NGFR rabbit IgG(H+L) / donkey NGFR 179.23
Colo858-A2-rep1
C3
COLO 858 Vemurafenib Belinostat 0.3160 2.6667
uM
 48
hr
 1 209 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 309.25 NGFR rabbit IgG(H+L) / donkey NGFR 21.49
Colo858-A2-rep1
C4
COLO 858 Vemurafenib Selisistat 0.3160 2.6667
uM
 48
hr
 1 3015 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 359.72 NGFR rabbit IgG(H+L) / donkey NGFR 105.69
Colo858-A2-rep1
C5
COLO 858 Vemurafenib GSK-J2 0.3160 2.6667
uM
 48
hr
 1 2652 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 335.90 NGFR rabbit IgG(H+L) / donkey NGFR 155.80
Colo858-A2-rep1
C6
COLO 858 Vemurafenib (+)-JQ1 0.3160 2.6667
uM
 48
hr
 1 1745 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 123.39 NGFR rabbit IgG(H+L) / donkey NGFR 10.46
Colo858-A2-rep1
C7
COLO 858 Vemurafenib UNC1215 0.3160 2.6667
uM
 48
hr
 1 2700 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 375.51 NGFR rabbit IgG(H+L) / donkey NGFR 134.85
Colo858-A2-rep1
C8
COLO 858 Vemurafenib Vorinostat 0.3160 2.6667
uM
 48
hr
 1 329 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 200.63 NGFR rabbit IgG(H+L) / donkey NGFR 17.50
Colo858-A2-rep1
D2
COLO 858 Vemurafenib JW55 0.3160 2.6667
uM
 48
hr
 1 2243 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 243.79 NGFR rabbit IgG(H+L) / donkey NGFR 100.13
Colo858-A2-rep1
D3
COLO 858 Vemurafenib Entinostat 0.3160 2.6667
uM
 48
hr
 1 2256 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 128.46 NGFR rabbit IgG(H+L) / donkey NGFR 15.79
Colo858-A2-rep1
D4
COLO 858 Vemurafenib AGK2 0.3160 2.6667
uM
 48
hr
 1 564 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 311.41 NGFR rabbit IgG(H+L) / donkey NGFR 108.97
Colo858-A2-rep1
D5
COLO 858 Vemurafenib GSK-J4 0.3160 2.6667
uM
 48
hr
 1 528 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 232.59 NGFR rabbit IgG(H+L) / donkey NGFR 14.66
Colo858-A2-rep1
D6
COLO 858 Vemurafenib (-)-JQ1 0.3160 2.6667
uM
 48
hr
 1 3175 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 309.67 NGFR rabbit IgG(H+L) / donkey NGFR 140.73
Colo858-A2-rep1
D7
COLO 858 Vemurafenib IOX2 0.3160 2.6667
uM
 48
hr
 1 3424 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 351.29 NGFR rabbit IgG(H+L) / donkey NGFR 150.84
Colo858-A2-rep1
D8
COLO 858 Vemurafenib Azacitidine 0.3160 2.6667
uM
 48
hr
 1 840 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 198.97 NGFR rabbit IgG(H+L) / donkey NGFR 20.08
Colo858-A2-rep1
E2
COLO 858 Vemurafenib C646 0.3160 2.6667
uM
 48
hr
 1 2689 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 316.57 NGFR rabbit IgG(H+L) / donkey NGFR 154.25
Colo858-A2-rep1
E3
COLO 858 Vemurafenib Mocetinostat 0.3160 2.6667
uM
 48
hr
 1 2109 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 155.83 NGFR rabbit IgG(H+L) / donkey NGFR 22.08
Colo858-A2-rep1
E4