Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib JW55 1.0000 2.6667
uM
 48
hr
 1 2571 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 135.73 NGFR rabbit IgG(H+L) / donkey NGFR 138.78
A375-A2-rep1
D3
A-375 Vemurafenib Entinostat 1.0000 2.6667
uM
 48
hr
 1 2292 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 451.09 NGFR rabbit IgG(H+L) / donkey NGFR 264.91
A375-A2-rep1
D4
A-375 Vemurafenib AGK2 1.0000 2.6667
uM
 48
hr
 1 678 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 394.51 NGFR rabbit IgG(H+L) / donkey NGFR 132.96
A375-A2-rep1
D5
A-375 Vemurafenib GSK-J4 1.0000 2.6667
uM
 48
hr
 1 1533 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 141.60 NGFR rabbit IgG(H+L) / donkey NGFR 96.94
A375-A2-rep1
D6
A-375 Vemurafenib (-)-JQ1 1.0000 2.6667
uM
 48
hr
 1 3035 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 159.74 NGFR rabbit IgG(H+L) / donkey NGFR 239.66
A375-A2-rep1
D7
A-375 Vemurafenib IOX2 1.0000 2.6667
uM
 48
hr
 1 2950 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 154.83 NGFR rabbit IgG(H+L) / donkey NGFR 183.99
A375-A2-rep1
D8
A-375 Vemurafenib Azacitidine 1.0000 2.6667
uM
 48
hr
 1 1210 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 261.75 NGFR rabbit IgG(H+L) / donkey NGFR 53.65
A375-A2-rep1
E2
A-375 Vemurafenib C646 1.0000 2.6667
uM
 48
hr
 1 2172 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 156.52 NGFR rabbit IgG(H+L) / donkey NGFR 184.53
A375-A2-rep1
E3
A-375 Vemurafenib Mocetinostat 1.0000 2.6667
uM
 48
hr
 1 304 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 299.57 NGFR rabbit IgG(H+L) / donkey NGFR 87.98
A375-A2-rep1
E4
A-375 Vemurafenib Resveratrol 1.0000 2.6667
uM
 48
hr
 1 2505 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 181.88 NGFR rabbit IgG(H+L) / donkey NGFR 186.11
A375-A2-rep1
E5
A-375 Vemurafenib Daminozide 1.0000 2.6667
uM
 48
hr
 1 2676 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 168.16 NGFR rabbit IgG(H+L) / donkey NGFR 205.28
A375-A2-rep1
E6
A-375 Vemurafenib I-BET 1.0000 2.6667
uM
 48
hr
 1 2375 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 130.55 NGFR rabbit IgG(H+L) / donkey NGFR 81.67
A375-A2-rep1
E7
A-375 Vemurafenib Epigallocatechin gallate 1.0000 2.6667
uM
 48
hr
 1 2367 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 176.81 NGFR rabbit IgG(H+L) / donkey NGFR 170.27
A375-A2-rep1
E8
A-375 Vemurafenib Decitabine 1.0000 2.6667
uM
 48
hr
 1 1969 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 193.22 NGFR rabbit IgG(H+L) / donkey NGFR 150.60
A375-A2-rep1
F2
A-375 Vemurafenib Garcinol 1.0000 2.6667
uM
 48
hr
 1 1325 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 146.88 NGFR rabbit IgG(H+L) / donkey NGFR 175.82
A375-A2-rep1
F3
A-375 Vemurafenib Pracinostat 1.0000 2.6667
uM
 48
hr
 1 137 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 388.80 NGFR rabbit IgG(H+L) / donkey NGFR 39.73
A375-A2-rep1
F4
A-375 Vemurafenib BIX-01294 1.0000 2.6667
uM
 48
hr
 1 825 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 295.68 NGFR rabbit IgG(H+L) / donkey NGFR 33.17
A375-A2-rep1
F5
A-375 Vemurafenib Methylstat 1.0000 2.6667
uM
 48
hr
 1 1079 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 150.72 NGFR rabbit IgG(H+L) / donkey NGFR 55.48
A375-A2-rep1
F6
A-375 Vemurafenib I-BET151 1.0000 2.6667
uM
 48
hr
 1 1612 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 124.73 NGFR rabbit IgG(H+L) / donkey NGFR 68.89
A375-A2-rep1
F7
A-375 Vemurafenib CX-5461 1.0000 2.6667
uM
 48
hr
 1 1229 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 146.16 NGFR rabbit IgG(H+L) / donkey NGFR 147.71
A375-A2-rep1
F8
A-375 Vemurafenib RG-108 1.0000 2.6667
uM
 48
hr
 1 1476 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 171.86 NGFR rabbit IgG(H+L) / donkey NGFR 162.39
A375-A2-rep1
G2
A-375 Vemurafenib Anacardic acid 1.0000 2.6667
uM
 48
hr
 1 1318 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 145.31 NGFR rabbit IgG(H+L) / donkey NGFR 170.13
A375-A2-rep1
G3
A-375 Vemurafenib MC1568 1.0000 2.6667
uM
 48
hr
 1 1904 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 389.68 NGFR rabbit IgG(H+L) / donkey NGFR 146.07
A375-A2-rep1
G4
A-375 Vemurafenib UNC0638 1.0000 2.6667
uM
 48
hr
 1 1266 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 181.34 NGFR rabbit IgG(H+L) / donkey NGFR 57.07
A375-A2-rep1
G5
A-375 Vemurafenib Tranylcypromine 1.0000 2.6667
uM
 48
hr
 1 1440 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 151.07 NGFR rabbit IgG(H+L) / donkey NGFR 215.58
A375-A2-rep1
G6