Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib Ischemin 1.0000 2.6667
uM
 48
hr
 1 1528 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 168.95 NGFR rabbit IgG(H+L) / donkey NGFR 198.54
A375-A2-rep1
G7
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 1088 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 136.47 NGFR rabbit IgG(H+L) / donkey NGFR 155.22
A375-A2-rep1
H10
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 1210 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 152.35 NGFR rabbit IgG(H+L) / donkey NGFR 155.16
A375-A2-rep1
H11
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 1206 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 152.77 NGFR rabbit IgG(H+L) / donkey NGFR 156.70
A375-A2-rep1
H2
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 1034 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 152.56 NGFR rabbit IgG(H+L) / donkey NGFR 144.52
A375-A2-rep1
H3
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 1004 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 142.24 NGFR rabbit IgG(H+L) / donkey NGFR 122.61
A375-A2-rep1
H4
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 955 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 144.67 NGFR rabbit IgG(H+L) / donkey NGFR 131.81
A375-A2-rep1
H5
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 969 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 161.13 NGFR rabbit IgG(H+L) / donkey NGFR 146.47
A375-A2-rep1
H6
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 1074 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 168.31 NGFR rabbit IgG(H+L) / donkey NGFR 161.00
A375-A2-rep1
H7
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 1099 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 168.81 NGFR rabbit IgG(H+L) / donkey NGFR 136.50
A375-A2-rep1
H8
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 1087 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 149.27 NGFR rabbit IgG(H+L) / donkey NGFR 138.42
A375-A2-rep1
H9
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1915 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 204.90 NGFR rabbit IgG(H+L) / donkey NGFR 199.36
A375-A2-rep2
A10
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1793 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 164.73 NGFR rabbit IgG(H+L) / donkey NGFR 197.31
A375-A2-rep2
A11
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 2412 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 231.59 NGFR rabbit IgG(H+L) / donkey NGFR 226.51
A375-A2-rep2
A2
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 2177 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 175.69 NGFR rabbit IgG(H+L) / donkey NGFR 218.26
A375-A2-rep2
A3
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 2355 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 209.47 NGFR rabbit IgG(H+L) / donkey NGFR 230.86
A375-A2-rep2
A4
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 2194 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 194.15 NGFR rabbit IgG(H+L) / donkey NGFR 186.08
A375-A2-rep2
A5
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 2033 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 217.52 NGFR rabbit IgG(H+L) / donkey NGFR 238.98
A375-A2-rep2
A6
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1797 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 197.36 NGFR rabbit IgG(H+L) / donkey NGFR 224.46
A375-A2-rep2
A7
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1871 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 184.59 NGFR rabbit IgG(H+L) / donkey NGFR 210.13
A375-A2-rep2
A8
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 2409 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 178.97 NGFR rabbit IgG(H+L) / donkey NGFR 199.27
A375-A2-rep2
A9
A-375 Vemurafenib Olaparib 1.0000 2.6667
uM
 48
hr
 2 2229 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 159.21 NGFR rabbit IgG(H+L) / donkey NGFR 210.77
A375-A2-rep2
B2
A-375 Vemurafenib Iniparib 1.0000 2.6667
uM
 48
hr
 2 2597 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 177.05 NGFR rabbit IgG(H+L) / donkey NGFR 176.99
A375-A2-rep2
B3
A-375 Vemurafenib CTB 1.0000 2.6667
uM
 48
hr
 2 2634 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 183.35 NGFR rabbit IgG(H+L) / donkey NGFR 163.76
A375-A2-rep2
B4
A-375 Vemurafenib Rocilinostat 1.0000 2.6667
uM
 48
hr
 2 2940 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 277.54 NGFR rabbit IgG(H+L) / donkey NGFR 76.23
A375-A2-rep2
B5