Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib Azacitidine 1.0000 0.1067
uM
 48
hr
 1 2464 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 196.92 NGFR rabbit IgG(H+L) / donkey NGFR 145.59
A375-B2-rep1
E2
A-375 Vemurafenib C646 1.0000 0.1067
uM
 48
hr
 1 2669 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 187.99 NGFR rabbit IgG(H+L) / donkey NGFR 202.06
A375-B2-rep1
E3
A-375 Vemurafenib Mocetinostat 1.0000 0.1067
uM
 48
hr
 1 2929 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 280.55 NGFR rabbit IgG(H+L) / donkey NGFR 517.04
A375-B2-rep1
E4
A-375 Vemurafenib Resveratrol 1.0000 0.1067
uM
 48
hr
 1 2946 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 177.17 NGFR rabbit IgG(H+L) / donkey NGFR 188.00
A375-B2-rep1
E5
A-375 Vemurafenib Daminozide 1.0000 0.1067
uM
 48
hr
 1 3275 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 183.42 NGFR rabbit IgG(H+L) / donkey NGFR 211.84
A375-B2-rep1
E6
A-375 Vemurafenib I-BET 1.0000 0.1067
uM
 48
hr
 1 3036 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 143.71 NGFR rabbit IgG(H+L) / donkey NGFR 197.93
A375-B2-rep1
E7
A-375 Vemurafenib Epigallocatechin gallate 1.0000 0.1067
uM
 48
hr
 1 2916 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 172.25 NGFR rabbit IgG(H+L) / donkey NGFR 188.44
A375-B2-rep1
E8
A-375 Vemurafenib Decitabine 1.0000 0.1067
uM
 48
hr
 1 2198 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 190.33 NGFR rabbit IgG(H+L) / donkey NGFR 172.41
A375-B2-rep1
F2
A-375 Vemurafenib Garcinol 1.0000 0.1067
uM
 48
hr
 1 2418 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 177.58 NGFR rabbit IgG(H+L) / donkey NGFR 200.14
A375-B2-rep1
F3
A-375 Vemurafenib Pracinostat 1.0000 0.1067
uM
 48
hr
 1 2816 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 487.81 NGFR rabbit IgG(H+L) / donkey NGFR 126.79
A375-B2-rep1
F4
A-375 Vemurafenib BIX-01294 1.0000 0.1067
uM
 48
hr
 1 2703 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 173.26 NGFR rabbit IgG(H+L) / donkey NGFR 174.07
A375-B2-rep1
F5
A-375 Vemurafenib Methylstat 1.0000 0.1067
uM
 48
hr
 1 2624 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 155.41 NGFR rabbit IgG(H+L) / donkey NGFR 164.98
A375-B2-rep1
F6
A-375 Vemurafenib I-BET151 1.0000 0.1067
uM
 48
hr
 1 2693 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 165.02 NGFR rabbit IgG(H+L) / donkey NGFR 202.21
A375-B2-rep1
F7
A-375 Vemurafenib CX-5461 1.0000 0.1067
uM
 48
hr
 1 1884 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 133.96 NGFR rabbit IgG(H+L) / donkey NGFR 170.61
A375-B2-rep1
F8
A-375 Vemurafenib RG-108 1.0000 0.1067
uM
 48
hr
 1 2074 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 144.70 NGFR rabbit IgG(H+L) / donkey NGFR 138.05
A375-B2-rep1
G2
A-375 Vemurafenib Anacardic acid 1.0000 0.1067
uM
 48
hr
 1 1913 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 163.69 NGFR rabbit IgG(H+L) / donkey NGFR 176.01
A375-B2-rep1
G3
A-375 Vemurafenib MC1568 1.0000 0.1067
uM
 48
hr
 1 2089 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 205.11 NGFR rabbit IgG(H+L) / donkey NGFR 176.85
A375-B2-rep1
G4
A-375 Vemurafenib UNC0638 1.0000 0.1067
uM
 48
hr
 1 1988 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 183.41 NGFR rabbit IgG(H+L) / donkey NGFR 148.89
A375-B2-rep1
G5
A-375 Vemurafenib Tranylcypromine 1.0000 0.1067
uM
 48
hr
 1 2043 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 164.99 NGFR rabbit IgG(H+L) / donkey NGFR 192.09
A375-B2-rep1
G6
A-375 Vemurafenib Ischemin 1.0000 0.1067
uM
 48
hr
 1 1998 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 186.72 NGFR rabbit IgG(H+L) / donkey NGFR 161.90
A375-B2-rep1
G7
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 1702 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 164.33 NGFR rabbit IgG(H+L) / donkey NGFR 152.80
A375-B2-rep1
H10
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 1693 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 154.82 NGFR rabbit IgG(H+L) / donkey NGFR 130.50
A375-B2-rep1
H11
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 1685 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 150.63 NGFR rabbit IgG(H+L) / donkey NGFR 141.16
A375-B2-rep1
H2
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 1675 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 168.32 NGFR rabbit IgG(H+L) / donkey NGFR 154.64
A375-B2-rep1
H3
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 1748 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 166.30 NGFR rabbit IgG(H+L) / donkey NGFR 148.34
A375-B2-rep1
H4