Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib Epigallocatechin gallate 0.3160 0.5333
uM
 48
hr
 1 2951 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 343.41 NGFR rabbit IgG(H+L) / donkey NGFR 153.52
Colo858-B1-rep1
E8
COLO 858 Vemurafenib Decitabine 0.3160 0.5333
uM
 48
hr
 1 1919 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 251.82 NGFR rabbit IgG(H+L) / donkey NGFR 160.06
Colo858-B1-rep1
F2
COLO 858 Vemurafenib Garcinol 0.3160 0.5333
uM
 48
hr
 1 2044 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 287.68 NGFR rabbit IgG(H+L) / donkey NGFR 135.93
Colo858-B1-rep1
F3
COLO 858 Vemurafenib Pracinostat 0.3160 0.5333
uM
 48
hr
 1 456 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 184.16 NGFR rabbit IgG(H+L) / donkey NGFR 34.51
Colo858-B1-rep1
F4
COLO 858 Vemurafenib BIX-01294 0.3160 0.5333
uM
 48
hr
 1 2472 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 382.91 NGFR rabbit IgG(H+L) / donkey NGFR 124.85
Colo858-B1-rep1
F5
COLO 858 Vemurafenib Methylstat 0.3160 0.5333
uM
 48
hr
 1 2664 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 266.70 NGFR rabbit IgG(H+L) / donkey NGFR 133.40
Colo858-B1-rep1
F6
COLO 858 Vemurafenib I-BET151 0.3160 0.5333
uM
 48
hr
 1 1798 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 126.05 NGFR rabbit IgG(H+L) / donkey NGFR 16.08
Colo858-B1-rep1
F7
COLO 858 Vemurafenib CX-5461 0.3160 0.5333
uM
 48
hr
 1 2471 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 233.62 NGFR rabbit IgG(H+L) / donkey NGFR 207.52
Colo858-B1-rep1
F8
COLO 858 Vemurafenib RG-108 0.3160 0.5333
uM
 48
hr
 1 1663 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 305.65 NGFR rabbit IgG(H+L) / donkey NGFR 146.79
Colo858-B1-rep1
G2
COLO 858 Vemurafenib Anacardic acid 0.3160 0.5333
uM
 48
hr
 1 1751 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 322.89 NGFR rabbit IgG(H+L) / donkey NGFR 153.60
Colo858-B1-rep1
G3
COLO 858 Vemurafenib MC1568 0.3160 0.5333
uM
 48
hr
 1 1821 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 320.92 NGFR rabbit IgG(H+L) / donkey NGFR 121.48
Colo858-B1-rep1
G4
COLO 858 Vemurafenib UNC0638 0.3160 0.5333
uM
 48
hr
 1 1734 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 303.53 NGFR rabbit IgG(H+L) / donkey NGFR 201.94
Colo858-B1-rep1
G5
COLO 858 Vemurafenib Tranylcypromine 0.3160 0.5333
uM
 48
hr
 1 1762 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 318.48 NGFR rabbit IgG(H+L) / donkey NGFR 157.43
Colo858-B1-rep1
G6
COLO 858 Vemurafenib Ischemin 0.3160 0.5333
uM
 48
hr
 1 1952 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 337.98 NGFR rabbit IgG(H+L) / donkey NGFR 129.76
Colo858-B1-rep1
G7
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1172 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 265.99 NGFR rabbit IgG(H+L) / donkey NGFR 115.59
Colo858-B1-rep1
H10
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1009 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 308.25 NGFR rabbit IgG(H+L) / donkey NGFR 142.85
Colo858-B1-rep1
H11
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1335 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 315.51 NGFR rabbit IgG(H+L) / donkey NGFR 136.29
Colo858-B1-rep1
H2
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1171 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 341.30 NGFR rabbit IgG(H+L) / donkey NGFR 131.95
Colo858-B1-rep1
H3
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1070 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 308.39 NGFR rabbit IgG(H+L) / donkey NGFR 124.26
Colo858-B1-rep1
H4
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1132 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 307.65 NGFR rabbit IgG(H+L) / donkey NGFR 141.85
Colo858-B1-rep1
H5
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1037 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 280.57 NGFR rabbit IgG(H+L) / donkey NGFR 127.15
Colo858-B1-rep1
H6
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1122 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 307.07 NGFR rabbit IgG(H+L) / donkey NGFR 147.66
Colo858-B1-rep1
H7
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1014 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 285.27 NGFR rabbit IgG(H+L) / donkey NGFR 120.90
Colo858-B1-rep1
H8
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1265 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 315.56 NGFR rabbit IgG(H+L) / donkey NGFR 114.69
Colo858-B1-rep1
H9
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 1231 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 346.46 NGFR rabbit IgG(H+L) / donkey NGFR 151.76
Colo858-B1-rep2
A10