Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

 Full screen view
Filter results: >>>>
Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib Pictilisib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 650.79 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 174.72 NGFR rabbit IgG(H+L) / donkey NGFR 490.71
A375-P7
D5
A-375 Vemurafenib AZD8055 1.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 745.76 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 173.12 NGFR rabbit IgG(H+L) / donkey NGFR 644.03
A375-P7
D6
A-375 Vemurafenib AZD8055 1.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 684.78 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 167.37 NGFR rabbit IgG(H+L) / donkey NGFR 532.87
A375-P7
D7
A-375 Vemurafenib JNK-IN-8 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 345.98 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 120.79 NGFR rabbit IgG(H+L) / donkey NGFR 369.72
A375-P7
D8
A-375 Vemurafenib JNK-IN-8 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 441.74 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 139.78 NGFR rabbit IgG(H+L) / donkey NGFR 262.27
A375-P7
D9
A-375 Vemurafenib Palbociclib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1109.41 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 193.91 NGFR rabbit IgG(H+L) / donkey NGFR 477.86
A375-P7
E10
A-375 Vemurafenib Palbociclib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 773.87 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 175.18 NGFR rabbit IgG(H+L) / donkey NGFR 402.20
A375-P7
E11
A-375 Vemurafenib Saracatinib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2657.88 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 504.90 NGFR rabbit IgG(H+L) / donkey NGFR 261.89
A375-P7
E2
A-375 Vemurafenib Saracatinib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3009.25 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 525.64 NGFR rabbit IgG(H+L) / donkey NGFR 196.08
A375-P7
E3
A-375 Vemurafenib Pictilisib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2339.94 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 429.64 NGFR rabbit IgG(H+L) / donkey NGFR 360.80
A375-P7
E4
A-375 Vemurafenib Pictilisib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1566.16 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 332.72 NGFR rabbit IgG(H+L) / donkey NGFR 283.67
A375-P7
E5
A-375 Vemurafenib AZD8055 0.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1689.11 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 371.16 NGFR rabbit IgG(H+L) / donkey NGFR 370.45
A375-P7
E6
A-375 Vemurafenib AZD8055 0.000 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1607.03 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 317.34 NGFR rabbit IgG(H+L) / donkey NGFR 319.81
A375-P7
E7
A-375 Vemurafenib JNK-IN-8 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2413.32 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 408.16 NGFR rabbit IgG(H+L) / donkey NGFR 329.47
A375-P7
E8
A-375 Vemurafenib JNK-IN-8 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2087.72 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 373.66 NGFR rabbit IgG(H+L) / donkey NGFR 130.03
A375-P7
E9
A-375 Vemurafenib Palbociclib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 354.17 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 209.98 NGFR rabbit IgG(H+L) / donkey NGFR 635.87
A375-P7
F10
A-375 Vemurafenib Palbociclib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 290.36 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 112.32 NGFR rabbit IgG(H+L) / donkey NGFR 272.43
A375-P7
F11
A-375 Vemurafenib Saracatinib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 583.64 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 137.10 NGFR rabbit IgG(H+L) / donkey NGFR 286.84
A375-P7
F2
A-375 Vemurafenib Saracatinib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 707.83 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 161.86 NGFR rabbit IgG(H+L) / donkey NGFR 226.02
A375-P7
F3
A-375 Vemurafenib Pictilisib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 866.57 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 186.26 NGFR rabbit IgG(H+L) / donkey NGFR 661.46
A375-P7
F4
A-375 Vemurafenib Pictilisib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 882.31 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 203.27 NGFR rabbit IgG(H+L) / donkey NGFR 381.84
A375-P7
F5
A-375 Vemurafenib AZD8055 0.316 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1065.67 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 221.73 NGFR rabbit IgG(H+L) / donkey NGFR 534.00
A375-P7
F6
A-375 Vemurafenib AZD8055 0.316 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 989.80 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 212.12 NGFR rabbit IgG(H+L) / donkey NGFR 424.21
A375-P7
F7
A-375 Vemurafenib JNK-IN-8 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 439.99 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 126.76 NGFR rabbit IgG(H+L) / donkey NGFR 270.31
A375-P7
F8
A-375 Vemurafenib JNK-IN-8 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 563.89 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 174.96 NGFR rabbit IgG(H+L) / donkey NGFR 185.32
A375-P7
F9