Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib Palbociclib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1006.86 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 202.69 NGFR rabbit IgG(H+L) / donkey NGFR 483.20
A375-P7
B10
A-375 Vemurafenib Palbociclib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 673.31 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 179.29 NGFR rabbit IgG(H+L) / donkey NGFR 398.37
A375-P7
B11
A-375 Vemurafenib Saracatinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2781.07 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 536.04 NGFR rabbit IgG(H+L) / donkey NGFR 281.52
A375-P7
B2
A-375 Vemurafenib Saracatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3073.51 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 581.89 NGFR rabbit IgG(H+L) / donkey NGFR 205.60
A375-P7
B3
A-375 Vemurafenib Pictilisib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2523.29 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 466.73 NGFR rabbit IgG(H+L) / donkey NGFR 345.12
A375-P7
B4
A-375 Vemurafenib Pictilisib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1903.13 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 389.14 NGFR rabbit IgG(H+L) / donkey NGFR 273.51
A375-P7
B5
A-375 Vemurafenib AZD8055 0.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1911.21 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 394.21 NGFR rabbit IgG(H+L) / donkey NGFR 310.04
A375-P7
B6
A-375 Vemurafenib AZD8055 0.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1583.62 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 345.10 NGFR rabbit IgG(H+L) / donkey NGFR 297.21
A375-P7
B7
A-375 Vemurafenib JNK-IN-8 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2530.85 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 466.71 NGFR rabbit IgG(H+L) / donkey NGFR 357.38
A375-P7
B8
A-375 Vemurafenib JNK-IN-8 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2096.28 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 413.41 NGFR rabbit IgG(H+L) / donkey NGFR 143.89
A375-P7
B9
A-375 Vemurafenib Palbociclib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 472.08 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 159.91 NGFR rabbit IgG(H+L) / donkey NGFR 749.94
A375-P7
C10
A-375 Vemurafenib Palbociclib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 297.68 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 114.57 NGFR rabbit IgG(H+L) / donkey NGFR 358.16
A375-P7
C11
A-375 Vemurafenib Saracatinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 610.68 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 153.72 NGFR rabbit IgG(H+L) / donkey NGFR 258.83
A375-P7
C2
A-375 Vemurafenib Saracatinib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 630.31 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 169.48 NGFR rabbit IgG(H+L) / donkey NGFR 213.50
A375-P7
C3
A-375 Vemurafenib Pictilisib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 837.41 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 197.51 NGFR rabbit IgG(H+L) / donkey NGFR 583.37
A375-P7
C4
A-375 Vemurafenib Pictilisib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 786.90 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 197.62 NGFR rabbit IgG(H+L) / donkey NGFR 429.03
A375-P7
C5
A-375 Vemurafenib AZD8055 0.316 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1015.43 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 227.72 NGFR rabbit IgG(H+L) / donkey NGFR 506.01
A375-P7
C6
A-375 Vemurafenib AZD8055 0.316 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 979.73 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 221.80 NGFR rabbit IgG(H+L) / donkey NGFR 400.84
A375-P7
C7
A-375 Vemurafenib JNK-IN-8 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 413.43 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 139.34 NGFR rabbit IgG(H+L) / donkey NGFR 264.58
A375-P7
C8
A-375 Vemurafenib JNK-IN-8 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 477.45 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 147.57 NGFR rabbit IgG(H+L) / donkey NGFR 140.32
A375-P7
C9
A-375 Vemurafenib Palbociclib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 429.67 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 323.04 NGFR rabbit IgG(H+L) / donkey NGFR 785.11
A375-P7
D10
A-375 Vemurafenib Palbociclib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 443.96 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 189.38 NGFR rabbit IgG(H+L) / donkey NGFR 431.55
A375-P7
D11
A-375 Vemurafenib Saracatinib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 560.81 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 155.02 NGFR rabbit IgG(H+L) / donkey NGFR 343.48
A375-P7
D2
A-375 Vemurafenib Saracatinib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 614.51 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 174.13 NGFR rabbit IgG(H+L) / donkey NGFR 225.89
A375-P7
D3
A-375 Vemurafenib Pictilisib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 703.08 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 176.42 NGFR rabbit IgG(H+L) / donkey NGFR 642.42
A375-P7
D4