Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib Pictilisib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 542.42 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 186.55 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 217.56
A375-P10
C5
A-375 Vemurafenib AZD8055 0.316 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 892.27 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 227.01 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 314.05
A375-P10
C6
A-375 Vemurafenib AZD8055 0.316 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 737.85 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 204.50 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 267.04
A375-P10
C7
A-375 Vemurafenib JNK-IN-8 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 413.18 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 160.47 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 115.79
A375-P10
C8
A-375 Vemurafenib JNK-IN-8 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 411.11 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 156.52 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 84.74
A375-P10
C9
A-375 Vemurafenib Palbociclib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 246.62 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 114.97 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 222.09
A375-P10
D10
A-375 Vemurafenib Palbociclib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 150.26 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 90.18 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 169.63
A375-P10
D11
A-375 Vemurafenib Saracatinib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 532.78 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 182.44 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 261.07
A375-P10
D2
A-375 Vemurafenib Saracatinib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 485.65 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 175.90 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 226.89
A375-P10
D3
A-375 Vemurafenib Pictilisib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 488.77 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 173.57 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 268.96
A375-P10
D4
A-375 Vemurafenib Pictilisib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 480.09 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 167.47 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 215.99
A375-P10
D5
A-375 Vemurafenib AZD8055 1.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 534.00 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 177.96 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 299.97
A375-P10
D6
A-375 Vemurafenib AZD8055 1.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 695.94 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 192.03 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 280.62
A375-P10
D7
A-375 Vemurafenib JNK-IN-8 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 255.97 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 133.31 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 106.28
A375-P10
D8
A-375 Vemurafenib JNK-IN-8 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 369.03 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 139.31 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 75.47
A375-P10
D9
A-375 Vemurafenib Palbociclib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 900.98 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 182.23 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 132.24
A375-P10
E10
A-375 Vemurafenib Palbociclib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 560.61 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 140.38 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 108.53
A375-P10
E11
A-375 Vemurafenib Saracatinib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2223.28 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 406.34 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 214.00
A375-P10
E2
A-375 Vemurafenib Saracatinib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2231.52 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 405.65 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 204.22
A375-P10
E3
A-375 Vemurafenib Pictilisib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1868.43 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 337.91 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 200.07
A375-P10
E4
A-375 Vemurafenib Pictilisib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1121.62 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 267.34 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 152.07
A375-P10
E5
A-375 Vemurafenib AZD8055 0.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1301.07 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 298.21 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 167.81
A375-P10
E6
A-375 Vemurafenib AZD8055 0.000 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1255.45 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 286.02 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 186.22
A375-P10
E7
A-375 Vemurafenib JNK-IN-8 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2061.35 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 372.92 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 142.91
A375-P10
E8
A-375 Vemurafenib JNK-IN-8 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1763.17 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 339.26 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 130.86
A375-P10
E9