Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib Trametinib 0.316 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 292.18 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 133.03 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 807.25
A375-P3
F5
A-375 Vemurafenib Defactinib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 407.98 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 135.39 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 470.31
A375-P3
F6
A-375 Vemurafenib Defactinib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 356.57 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 130.47 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 402.10
A375-P3
F7
A-375 Vemurafenib PF562271 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 393.59 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 134.83 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 503.03
A375-P3
F8
A-375 Vemurafenib PF562271 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 280.58 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 133.67 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 278.95
A375-P3
F9
A-375 Vemurafenib Dasatinib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 385.73 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 151.89 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 363.22
A375-P3
G10
A-375 Vemurafenib Dasatinib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 362.39 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 142.43 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 326.27
A375-P3
G11
A-375 Vemurafenib 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 494.53 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 129.01 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 542.75
A375-P3
G2
A-375 Vemurafenib 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 475.50 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 144.00 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 530.78
A375-P3
G3
A-375 Vemurafenib Trametinib 1.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 367.34 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 145.41 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 738.84
A375-P3
G4
A-375 Vemurafenib Trametinib 1.000 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 386.85 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 146.10 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 798.02
A375-P3
G5
A-375 Vemurafenib Defactinib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 379.23 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 124.65 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 402.65
A375-P3
G6
A-375 Vemurafenib Defactinib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 391.87 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 132.61 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 397.86
A375-P3
G7
A-375 Vemurafenib PF562271 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 391.19 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 127.30 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 388.14
A375-P3
G8
A-375 Vemurafenib PF562271 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 350.60 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 131.47 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 244.17
A375-P3
G9
A-375 Vemurafenib Dasatinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2181.70 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 406.40 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 169.81
A375-P4
B10
A-375 Vemurafenib Dasatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1686.27 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 314.79 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 140.38
A375-P4
B11
A-375 Vemurafenib 0.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2597.12 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 404.96 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 265.99
A375-P4
B2
A-375 Vemurafenib 0.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2409.02 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 391.32 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 254.09
A375-P4
B3
A-375 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 230.23 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 126.27 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 293.14
A375-P4
B4
A-375 Vemurafenib Trametinib 0.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 260.65 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 134.67 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 350.66
A375-P4
B5
A-375 Vemurafenib Defactinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2059.99 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 371.47 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 198.06
A375-P4
B6
A-375 Vemurafenib Defactinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1527.20 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 297.11 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 183.05
A375-P4
B7
A-375 Vemurafenib PF562271 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1984.98 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 378.90 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 182.56
A375-P4
B8
A-375 Vemurafenib PF562271 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 787.92 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 188.07 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 135.23
A375-P4
B9