Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib Pictilisib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 589.31 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 160.08 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 343.41
A375-P9
G5
A-375 Vemurafenib AZD8055 1.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 709.09 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 175.17 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 490.12
A375-P9
G6
A-375 Vemurafenib AZD8055 1.000 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 672.99 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 167.19 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 410.14
A375-P9
G7
A-375 Vemurafenib JNK-IN-8 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 326.16 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 102.57 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 297.95
A375-P9
G8
A-375 Vemurafenib JNK-IN-8 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 379.81 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 117.13 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 256.19
A375-P9
G9
COLO 858 Vemurafenib Dasatinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3684.23 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 505.69 NGFR rabbit IgG(H+L) / donkey NGFR 115.96
Colo858-P1
B10
COLO 858 Vemurafenib Dasatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3387.57 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 414.22 NGFR rabbit IgG(H+L) / donkey NGFR 87.43
Colo858-P1
B11
COLO 858 Vemurafenib 0.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2329.97 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 322.63 NGFR rabbit IgG(H+L) / donkey NGFR 125.82
Colo858-P1
B2
COLO 858 Vemurafenib 0.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2284.95 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 320.51 NGFR rabbit IgG(H+L) / donkey NGFR 111.17
Colo858-P1
B3
COLO 858 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 698.58 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 216.69 NGFR rabbit IgG(H+L) / donkey NGFR 1288.95
Colo858-P1
B4
COLO 858 Vemurafenib Trametinib 0.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 612.50 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 172.45 NGFR rabbit IgG(H+L) / donkey NGFR 1229.93
Colo858-P1
B5
COLO 858 Vemurafenib Defactinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2425.70 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 337.27 NGFR rabbit IgG(H+L) / donkey NGFR 110.89
Colo858-P1
B6
COLO 858 Vemurafenib Defactinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1932.49 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 271.50 NGFR rabbit IgG(H+L) / donkey NGFR 103.07
Colo858-P1
B7
COLO 858 Vemurafenib PF562271 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2477.01 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 332.26 NGFR rabbit IgG(H+L) / donkey NGFR 93.85
Colo858-P1
B8
COLO 858 Vemurafenib PF562271 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2172.45 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 364.23 NGFR rabbit IgG(H+L) / donkey NGFR 74.98
Colo858-P1
B9
COLO 858 Vemurafenib Dasatinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 739.65 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 194.15 NGFR rabbit IgG(H+L) / donkey NGFR 54.21
Colo858-P1
C10
COLO 858 Vemurafenib Dasatinib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 953.32 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 216.44 NGFR rabbit IgG(H+L) / donkey NGFR 57.05
Colo858-P1
C11
COLO 858 Vemurafenib 0.316
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1303.68 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 244.16 NGFR rabbit IgG(H+L) / donkey NGFR 525.63
Colo858-P1
C2
COLO 858 Vemurafenib 0.316
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1181.89 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 235.96 NGFR rabbit IgG(H+L) / donkey NGFR 475.07
Colo858-P1
C3
COLO 858 Vemurafenib Trametinib 0.316 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 663.37 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 168.59 NGFR rabbit IgG(H+L) / donkey NGFR 1157.13
Colo858-P1
C4
COLO 858 Vemurafenib Trametinib 0.316 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 572.16 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 165.44 NGFR rabbit IgG(H+L) / donkey NGFR 1097.04
Colo858-P1
C5
COLO 858 Vemurafenib Defactinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 637.08 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 199.79 NGFR rabbit IgG(H+L) / donkey NGFR 134.41
Colo858-P1
C6
COLO 858 Vemurafenib Defactinib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 493.03 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 154.05 NGFR rabbit IgG(H+L) / donkey NGFR 86.95
Colo858-P1
C7
COLO 858 Vemurafenib PF562271 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 597.72 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 178.20 NGFR rabbit IgG(H+L) / donkey NGFR 290.02
Colo858-P1
C8
COLO 858 Vemurafenib PF562271 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 528.46 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 174.68 NGFR rabbit IgG(H+L) / donkey NGFR 115.86
Colo858-P1
C9