Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib Dasatinib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 337.51 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 141.47 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 415.59
A375-P3
D10
A-375 Vemurafenib Dasatinib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 428.90 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 155.70 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 377.11
A375-P3
D11
A-375 Vemurafenib 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 588.83 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 158.19 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 576.48
A375-P3
D2
A-375 Vemurafenib 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 497.08 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 152.13 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 556.24
A375-P3
D3
A-375 Vemurafenib Trametinib 1.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 346.07 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 142.60 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 856.27
A375-P3
D4
A-375 Vemurafenib Trametinib 1.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 240.55 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 132.44 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 931.16
A375-P3
D5
A-375 Vemurafenib Defactinib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 165.74 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 130.82 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 499.78
A375-P3
D6
A-375 Vemurafenib Defactinib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 216.94 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 129.80 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 468.99
A375-P3
D7
A-375 Vemurafenib PF562271 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 253.76 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 134.99 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 464.45
A375-P3
D8
A-375 Vemurafenib PF562271 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 318.27 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 137.65 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 268.61
A375-P3
D9
A-375 Vemurafenib Dasatinib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2483.07 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 422.64 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 223.37
A375-P3
E10
A-375 Vemurafenib Dasatinib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1514.69 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 313.01 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 156.02
A375-P3
E11
A-375 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2735.24 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 403.13 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 434.01
A375-P3
E2
A-375 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2623.48 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 402.51 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 432.33
A375-P3
E3
A-375 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 388.49 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 147.04 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 863.20
A375-P3
E4
A-375 Vemurafenib Trametinib 0.000 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 272.59 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 153.77 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 886.56
A375-P3
E5
A-375 Vemurafenib Defactinib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2087.74 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 364.28 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 315.96
A375-P3
E6
A-375 Vemurafenib Defactinib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1330.25 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 270.77 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 275.47
A375-P3
E7
A-375 Vemurafenib PF562271 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2137.32 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 362.84 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 255.30
A375-P3
E8
A-375 Vemurafenib PF562271 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1198.87 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 189.88 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 186.48
A375-P3
E9
A-375 Vemurafenib Dasatinib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 373.28 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 145.58 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 360.58
A375-P3
F10
A-375 Vemurafenib Dasatinib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 438.91 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 154.77 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 318.75
A375-P3
F11
A-375 Vemurafenib 0.316
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 837.07 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 165.98 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 588.81
A375-P3
F2
A-375 Vemurafenib 0.316
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 726.17 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 166.07 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 617.98
A375-P3
F3
A-375 Vemurafenib Trametinib 0.316 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 313.31 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 129.41 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 713.04
A375-P3
F4