Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 391.3 1
0002-0042
 496.41 42.05 229 59.11 0.824
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 292.6 1
0003-0056
 98.31 56.55 335 72.08 0.810
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 381.6 1
0004-0065
 935.15 65.24 324 68.43 0.870
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 474.7 1
0005-0075
 282.41 75.08 268 64.04 0.821
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 272.0 1
0006-0076
 711.31 75.89 364 71.60 0.892
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 408.5 1
0007-0083
 664.99 83.53 405 80.57 0.784
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 281.5 1
0008-0092
 730.56 92.42 348 71.01 0.867
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 527.2 1
0009-0092
 567.38 92.61 154 68.43 0.413
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 450.7 1
0010-0096
 262.76 96.42 254 69.26 0.665
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 280.0 1
0011-0100
 77.40 101.22 295 66.18 0.846
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 255.4 1
0012-0106
 712.96 105.19 322 72.33 0.774
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 589.4 1
0013-0107
 844.87 105.02 171 73.50 0.398
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 455.8 1
0014-0122
 896.06 121.71 302 66.77 0.851
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 356.1 1
0015-0194
 835.35 194.66 279 62.53 0.897
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 334.6 1
0016-0199
 185.62 199.26 102 39.21 0.834
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 492.9 1
0017-0225
 584.08 224.71 214 58.04 0.798
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 481.2 1
0018-0242
 806.26 241.74 215 62.04 0.702
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 396.8 1
0019-0268
 829.39 268.12 262 59.36 0.935
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 401.1 1
0020-0280
 887.64 280.48 237 60.28 0.820
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 407.0 1
0021-0288
 639.34 288.26 221 57.94 0.827
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 397.5 1
0022-0302
 32.27 302.53 235 64.18 0.717
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 420.9 1
0023-0306
 478.27 306.20 195 58.18 0.724
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 307.7 1
0024-0323
 657.51 324.42 284 64.18 0.866
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 384.1 1
0025-0343
 280.32 343.85 258 62.53 0.829
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 416.3 1
0026-0365
 47.51 365.09 214 54.87 0.893