Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 262.7 9
0114-0584
723.59 584.53 331 68.67 0.882
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 273.3 9
0115-0592
144.52 592.32 318 69.26 0.833
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 245.0 9
0116-0591
674.89 590.99 317 66.53 0.900
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 256.0 9
0117-0600
594.64 600.11 339 70.77 0.851
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 258.6 9
0118-0599
296.56 598.65 303 67.84 0.827
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 312.1 9
0119-0597
805.83 597.68 333 75.84 0.728
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 228.8 9
0120-0602
928.18 602.38 277 62.77 0.883
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 244.2 9
0121-0606
412.90 606.62 306 66.77 0.863
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 226.7 9
0122-0607
466.79 607.03 331 67.84 0.904
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 293.5 9
0123-0613
30.57 613.25 236 60.77 0.803
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 274.5 9
0124-0615
239.99 615.89 244 63.94 0.750
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 219.2 9
0125-0617
908.56 617.47 303 66.53 0.860
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 220.5 9
0126-0625
671.13 625.54 417 79.26 0.834
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 293.0 9
0127-0638
544.10 637.32 277 67.01 0.775
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 252.5 9
0128-0648
205.49 647.63 330 67.60 0.908
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 254.7 9
0129-0652
501.09 652.73 310 66.77 0.874
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 275.8 9
0130-0658
185.37 657.89 315 67.84 0.860
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 265.1 9
0131-0664
921.81 663.93 269 61.11 0.905
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 281.4 9
0132-0667
677.49 666.70 275 62.18 0.894
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 287.1 9
0133-0671
770.94 672.08 269 66.18 0.772
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 267.4 9
0134-0674
398.05 674.25 276 63.36 0.864
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 290.1 9
0135-0680
852.31 679.91 486 84.67 0.852
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 241.4 9
0136-0682
319.34 683.00 307 65.60 0.897
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 276.1 9
0137-0685
378.24 685.79 273 61.36 0.911
COLO 858 Vemurafenib
3.16
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 381.4 1
0001-0032
204.59 33.10 257 63.01 0.813