Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 239.1 1
0001-0030
113.89 29.84 299 67.60 0.822
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 272.1 1
0002-0029
168.91 28.72 294 66.18 0.843
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 372.3 1
0003-0033
263.30 34.12 210 55.36 0.861
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 303.7 1
0004-0039
463.46 39.70 310 65.36 0.912
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 255.8 1
0005-0040
504.82 41.10 293 68.67 0.781
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 305.7 1
0006-0040
720.01 40.44 289 75.74 0.633
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 655.0 1
0007-0041
803.55 41.85 444 87.50 0.729
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 455.5 1
0008-0042
418.82 41.04 267 61.11 0.898
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 576.5 1
0009-0042
87.75 42.02 158 48.63 0.840
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 411.2 1
0010-0045
289.33 45.56 376 71.94 0.913
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 357.7 1
0011-0044
589.42 44.68 234 56.28 0.928
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 385.4 1
0012-0045
693.34 45.35 223 54.87 0.931
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 409.0 1
0013-0042
652.67 42.21 177 52.63 0.803
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 417.4 1
0014-0047
771.72 47.09 254 59.36 0.906
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 327.2 1
0015-0051
172.16 51.15 276 61.94 0.904
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 331.8 1
0016-0050
534.61 50.03 323 66.77 0.910
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 388.2 1
0017-0053
219.63 53.47 374 71.60 0.917
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 332.6 1
0018-0052
134.85 52.43 234 56.53 0.920
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 513.1 1
0019-0058
657.13 57.83 342 70.77 0.858
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 298.8 1
0020-0061
258.75 61.24 390 73.60 0.905
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 323.1 1
0021-0063
335.34 62.34 338 68.43 0.907
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 392.9 1
0022-0062
630.45 62.65 266 64.28 0.809
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 412.0 1
0023-0061
68.12 60.80 231 55.11 0.956
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 498.1 1
0024-0069
569.59 69.83 433 81.26 0.824
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 310.8 1
0025-0071
383.06 70.47 307 65.36 0.903
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