Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 549.2 1
0027-0369
 350.54 368.34 227 62.87 0.722
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 364.9 1
0028-0364
 510.31 363.63 226 58.77 0.822
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 347.5 1
0029-0367
 623.42 366.98 274 63.60 0.851
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 484.3 1
0030-0365
 742.98 365.12 193 57.36 0.737
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 165.1 1
0031-0368
 147.46 368.35 242 61.36 0.808
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 440.1 1
0032-0373
 306.88 373.44 229 61.11 0.771
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 452.2 1
0033-0383
 675.69 383.47 240 63.11 0.757
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 326.3 1
0034-0389
 935.70 389.37 282 67.60 0.776
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 484.9 1
0035-0407
 210.27 407.15 209 58.77 0.760
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 377.1 1
0036-0414
 70.13 414.26 215 57.70 0.812
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 559.3 1
0037-0416
 890.73 415.95 225 57.70 0.849
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 373.1 1
0038-0438
 666.57 438.67 262 64.53 0.791
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 743.2 1
0039-0444
 858.60 443.31 109 75.98 0.237
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 572.1 1
0040-0457
 419.20 458.00 233 57.36 0.890
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 451.9 1
0041-0462
 730.98 462.08 237 61.94 0.776
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 562.4 1
0042-0498
 314.58 498.27 227 59.60 0.803
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 306.6 1
0043-0524
 563.97 524.23 303 66.18 0.869
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 437.4 1
0044-0527
 829.85 527.56 240 62.77 0.765
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 465.3 1
0045-0536
 719.53 536.54 205 58.18 0.761
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 347.4 1
0046-0539
 360.16 539.84 277 72.43 0.664
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 346.5 1
0047-0539
 688.61 539.21 272 60.77 0.926
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 507.7 1
0048-0541
 636.49 541.13 202 54.18 0.865
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 367.0 1
0049-0548
 12.47 547.60 261 61.36 0.871
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 445.7 1
0050-0558
 95.67 558.64 212 58.18 0.787
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 305.5 1
0051-0568
 648.28 568.31 304 68.18 0.822