Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 487.5 6
0157-0667
 131.53 667.22 214 54.77 0.896
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 224.8 6
0158-0685
 754.34 685.71 364 73.26 0.852
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 243.6 6
0159-0683
 214.80 683.17 312 65.01 0.928
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 274.3 6
0160-0691
 383.37 691.24 545 87.15 0.902
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 240.9 6
0161-0691
 921.86 691.84 327 67.01 0.915
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 226.1 6
0162-0695
 699.02 694.81 134 44.04 0.868
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 275.0 6
0163-0703
 335.59 703.62 544 86.91 0.905
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 241.3 6
0164-0697
 712.80 697.08 155 46.39 0.905
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 242.3 6
0165-0703
 115.04 703.15 344 69.01 0.908
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 264.1 6
0166-0708
 252.76 707.97 278 62.53 0.894
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 289.6 7
0001-0033
 92.68 32.93 251 60.18 0.871
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 248.7 7
0002-0044
 386.80 44.51 294 62.77 0.938
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 327.4 7
0003-0048
 477.35 48.14 278 66.67 0.786
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 294.8 7
0004-0060
 407.20 59.76 285 63.36 0.892
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 359.9 7
0005-0062
 678.12 62.81 233 55.36 0.956
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 331.2 7
0006-0083
 498.41 83.94 245 59.11 0.881
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 260.4 7
0007-0088
 102.28 88.41 272 64.28 0.827
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 508.2 7
0008-0090
 312.19 90.06 243 58.28 0.899
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 306.4 7
0009-0088
 857.80 88.23 243 58.77 0.884
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 359.0 7
0010-0091
 568.14 91.09 249 59.11 0.895
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 387.9 7
0011-0090
 922.36 90.77 285 69.36 0.745
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 270.2 7
0012-0096
 804.71 96.45 347 71.01 0.865
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 327.0 7
0013-0099
 159.09 99.23 396 76.91 0.841
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 342.2 7
0014-0101
 457.94 101.78 288 63.36 0.902
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 372.5 7
0015-0105
 635.09 105.00 234 59.36 0.835