Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 341.8 6
0132-0581
58.91 581.53 285 62.18 0.926
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 262.6 6
0133-0585
191.91 585.57 308 69.26 0.807
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 247.8 6
0134-0581
760.19 580.73 169 51.36 0.805
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 308.7 6
0135-0586
779.09 586.58 318 73.70 0.736
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 253.4 6
0136-0593
393.16 593.77 325 68.77 0.864
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 243.6 6
0137-0598
524.28 598.86 360 74.43 0.817
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 256.6 6
0138-0604
407.96 603.66 268 60.77 0.912
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 251.6 6
0139-0606
719.80 606.67 342 71.60 0.838
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 240.2 6
0140-0610
668.02 609.38 332 71.60 0.814
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 208.6 6
0141-0610
944.17 610.24 325 67.36 0.900
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 197.9 6
0142-0624
254.48 624.73 435 81.36 0.826
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 407.4 6
0143-0620
473.85 620.29 254 59.94 0.888
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 257.9 6
0144-0625
498.09 624.46 322 69.60 0.835
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 336.0 6
0145-0621
588.56 622.24 126 47.11 0.713
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 257.9 6
0146-0626
604.38 625.56 306 69.36 0.799
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 240.0 6
0147-0631
399.30 631.24 270 60.18 0.937
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 271.0 6
0148-0643
820.36 643.79 331 69.60 0.859
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 217.2 6
0149-0647
762.95 647.48 391 74.67 0.881
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 258.0 6
0150-0647
185.53 647.15 314 69.01 0.828
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 440.1 6
0151-0650
467.28 649.78 254 58.53 0.932
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 232.0 6
0152-0655
654.47 655.82 408 83.15 0.741
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 221.8 6
0153-0657
227.78 657.24 307 67.36 0.850
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 210.5 6
0154-0663
678.83 662.86 420 78.08 0.866
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 497.3 6
0155-0660
152.33 659.97 228 56.28 0.904
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 237.2 6
0156-0664
317.48 664.23 379 78.43 0.774