Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 378.5 7
0016-0119
523.81 119.55 222 58.18 0.824
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 393.4 7
0017-0126
686.97 125.86 193 53.94 0.834
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 242.1 7
0018-0126
88.93 126.97 301 67.01 0.842
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 312.1 7
0019-0132
641.72 132.74 273 63.60 0.848
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 247.6 7
0020-0131
188.37 132.20 306 72.77 0.726
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 336.5 7
0021-0135
281.43 134.70 262 61.60 0.868
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 226.2 7
0022-0140
900.77 139.76 353 73.26 0.827
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 340.9 7
0023-0140
559.10 140.54 300 64.43 0.908
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 359.4 7
0024-0146
372.83 146.27 317 70.18 0.809
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 215.2 7
0025-0153
132.29 153.66 301 66.18 0.864
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 255.3 7
0026-0155
245.36 155.87 315 67.84 0.860
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 264.6 7
0027-0154
331.26 154.32 290 63.94 0.891
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 269.3 7
0028-0159
830.05 159.13 536 87.26 0.885
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 251.4 7
0029-0163
779.09 162.97 296 65.60 0.864
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 338.1 7
0030-0173
445.86 172.75 269 62.87 0.855
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 257.5 7
0031-0180
31.92 180.27 332 72.43 0.795
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 239.6 7
0032-0182
770.41 182.44 294 64.77 0.881
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 413.6 7
0033-0201
821.36 201.88 239 57.94 0.895
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 390.2 7
0034-0203
154.35 202.98 230 56.53 0.905
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 445.3 7
0035-0230
658.69 229.95 258 61.36 0.861
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 234.3 7
0036-0236
902.31 237.44 323 75.50 0.712
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 498.0 7
0037-0240
807.18 241.35 115 44.53 0.729
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 310.6 7
0038-0265
518.79 264.69 312 69.26 0.817
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 470.9 7
0039-0265
544.89 264.71 246 64.18 0.750
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 388.1 7
0040-0268
690.63 268.72 226 56.87 0.878