Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1051.8 9
0006-0103
 476.99 103.18 279 61.36 0.931
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 447.8 9
0007-0106
 354.74 106.26 246 59.60 0.870
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 481.3 9
0008-0118
 570.68 118.15 240 60.53 0.823
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1198.2 9
0009-0145
 347.01 145.15 199 53.11 0.886
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 867.3 9
0010-0148
 694.70 149.05 194 59.36 0.692
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 427.6 9
0011-0150
 125.25 150.20 225 59.46 0.800
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 401.5 9
0012-0157
 97.26 157.47 266 59.11 0.957
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 397.6 9
0013-0183
 323.97 183.54 246 61.60 0.815
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1362.3 9
0014-0194
 251.35 194.46 224 57.36 0.856
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 387.1 9
0015-0206
 858.14 205.90 261 62.18 0.848
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 931.9 9
0016-0210
 14.17 209.59 216 55.60 0.878
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1275.8 9
0017-0211
 377.37 210.79 172 54.04 0.740
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1107.9 9
0018-0224
 388.18 223.96 199 56.53 0.783
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1033.3 9
0019-0235
 80.57 235.54 201 56.18 0.800
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 513.5 9
0020-0244
 687.08 243.72 274 63.36 0.858
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 2411.2 9
0021-0246
 299.45 245.54 343 72.43 0.822
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 441.6 9
0022-0255
 445.90 255.26 242 57.94 0.906
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 566.5 9
0023-0268
 478.14 267.59 245 58.77 0.891
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 373.2 9
0024-0306
 621.04 306.21 306 65.60 0.894
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 351.4 9
0025-0336
 263.52 336.65 255 77.50 0.534
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 498.3 9
0026-0338
 565.15 337.78 294 65.60 0.859
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 556.3 9
0027-0352
 423.01 352.32 230 57.36 0.879
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 480.2 9
0028-0359
 647.64 359.30 201 52.04 0.933
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 664.7 9
0029-0366
 121.04 366.56 347 72.08 0.839
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 436.6 9
0030-0371
 599.07 371.81 274 62.77 0.874