Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 619.1 9
0031-0392
 112.90 392.27 302 70.18 0.770
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 499.7 9
0032-0394
 544.89 393.62 265 87.64 0.434
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 430.0 9
0033-0396
 427.88 397.25 206 56.77 0.803
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 517.6 9
0034-0412
 642.60 412.11 280 69.26 0.734
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 886.4 9
0035-0416
 462.97 416.38 197 60.28 0.681
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 800.5 9
0036-0419
 369.72 418.83 238 60.53 0.816
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 390.5 9
0037-0428
 443.00 427.25 275 64.77 0.824
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 415.9 9
0038-0429
 515.38 429.72 271 61.70 0.895
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 355.0 9
0039-0440
 364.69 440.16 252 61.01 0.851
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 412.5 9
0040-0502
 687.24 501.40 247 61.36 0.825
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 408.2 9
0041-0505
 670.84 506.14 206 54.63 0.867
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 555.1 9
0042-0526
 480.04 525.65 299 66.77 0.843
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 455.3 9
0043-0560
 241.00 560.24 284 66.77 0.801
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1091.7 9
0044-0582
 19.55 581.95 287 64.53 0.866
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 784.5 9
0045-0581
 306.88 581.25 226 58.18 0.839
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 429.1 9
0046-0596
 783.57 595.75 266 63.84 0.820
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 814.9 9
0047-0601
 698.86 601.44 250 62.53 0.804
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 340.9 9
0048-0605
 942.34 604.53 260 67.01 0.728
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 333.8 9
0049-0613
 300.09 612.81 325 66.43 0.926
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 303.3 9
0050-0664
 878.63 665.31 280 63.60 0.870
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 1039.4 1
0001-0030
 113.89 29.84 299 67.60 0.822
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 1501.2 1
0002-0029
 168.91 28.72 294 66.18 0.843
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 5638.9 1
0003-0033
 263.30 34.12 210 55.36 0.861
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 1428.6 1
0004-0039
 463.46 39.70 310 65.36 0.912
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 1043.9 1
0005-0040
 504.82 41.10 293 68.67 0.781