Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 339.9 8
0044-0518
 406.70 519.39 142 45.21 0.873
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 534.7 8
0045-0536
 565.66 536.59 212 64.04 0.650
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 347.3 8
0046-0533
 407.92 532.20 168 48.63 0.893
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 366.4 8
0047-0534
 301.31 534.72 230 59.11 0.827
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1038.0 8
0048-0548
 192.92 547.90 260 61.36 0.868
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 576.4 8
0049-0556
 511.92 556.09 302 66.18 0.866
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 837.0 8
0050-0566
 299.97 566.75 193 53.60 0.844
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1088.6 8
0051-0580
 924.69 579.23 294 65.36 0.865
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 419.3 8
0052-0589
 270.62 588.33 268 63.11 0.845
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 8418.0 8
0053-0595
 880.07 594.80 355 73.36 0.829
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 353.8 8
0054-0610
 218.90 609.47 260 67.84 0.710
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 676.1 8
0055-0621
 146.69 622.02 198 57.11 0.763
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 471.8 8
0056-0621
 290.70 621.09 249 60.18 0.864
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 728.4 8
0057-0639
 577.61 640.06 225 62.53 0.723
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 446.0 8
0058-0657
 135.73 657.10 210 61.70 0.693
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 350.9 8
0059-0652
 674.40 652.07 277 69.36 0.724
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1000.7 8
0060-0666
 415.93 666.00 171 55.11 0.707
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 830.5 8
0061-0699
 370.97 699.10 183 51.70 0.860
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 572.6 8
0062-0701
 133.33 701.42 195 51.70 0.917
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 137.7 8
0063-0709
 842.07 709.27 198 51.70 0.931
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 336.5 9
0001-0034
 31.77 34.33 273 65.01 0.812
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 443.5 9
0002-0034
 411.40 35.09 286 64.77 0.857
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 349.6 9
0003-0040
 489.21 39.94 280 65.84 0.812
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 492.6 9
0004-0049
 727.83 49.69 258 61.11 0.868
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 6972.7 9
0005-0071
 43.56 71.40 286 64.28 0.870