Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
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| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Vemurafenib | COLO 858 | JNK-IN-8 | 5.0000 | uM | 24 | hr | | Ki-67 | 2211.10 | p-Rb(S807/S811) | 96.70 | p-cJun(S73) | 24.08 | 2 | Colo858-signal3 | G2 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 5.0000 | uM | 24 | hr | | p27 Kip1 | 598.80 | p-Rb(S807/S811) | 103.77 | p-ERK(T202/Y204) | 63.79 | 1 | LOXIMVI-signal1 | F2 |
| JNK-IN-8 | COLO 858 | 5.0000 | uM | 24 | hr | | Ki-67 | 2447.10 | p-Rb(S807/S811) | 231.66 | p-cJun(S73) | 37.00 | 1 | Colo858-signal3 | D2 | |
| Vemurafenib | COLO 858 | JNK-IN-8 | 5.0000 | uM | 24 | hr | | Ki-67 | 1967.00 | p-Rb(S807/S811) | 99.41 | p-cJun(S73) | 22.51 | 1 | Colo858-signal3 | F2 |
| JNK-IN-8 | LOXIMVI | 5.0000 | uM | 24 | hr | | p27 Kip1 | 464.53 | p-Rb(S807/S811) | 268.11 | p-ERK(T202/Y204) | 267.97 | 1 | LOXIMVI-signal1 | D2 | |
| JNK-IN-8 | LOXIMVI | 5.0000 | uM | 24 | hr | | p27 Kip1 | 472.18 | p-Rb(S807/S811) | 278.69 | p-ERK(T202/Y204) | 272.54 | 2 | LOXIMVI-signal1 | E2 | |
| Vemurafenib | COLO 858 | JNK-IN-8 | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 525.79 | p-Rb(S807/S811) | 128.17 | p-S6(S235/S236 | 40.46 | 2 | Colo858-signal2 | G2 |
| Vemurafenib | COLO 858 | 5.0000 | uM | 24 | hr | | Ki-67 | 947.93 | p-Rb(S807/S811) | 51.31 | p-cJun(S73) | 41.01 | 2 | Colo858-signal3 | C2 | |
| Vemurafenib | COLO 858 | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 265.46 | p-Rb(S807/S811) | 66.87 | p-S6(S235/S236 | 14.48 | 1 | Colo858-signal2 | B2 | |
| JNK-IN-8 | COLO 858 | 5.0000 | uM | 24 | hr | | Ki-67 | 2634.30 | p-Rb(S807/S811) | 252.91 | p-cJun(S73) | 39.50 | 2 | Colo858-signal3 | E2 | |
| Vemurafenib | LOXIMVI | 5.0000 | uM | 24 | hr | | p27 Kip1 | 499.44 | p-Rb(S807/S811) | 81.11 | p-ERK(T202/Y204) | 31.87 | 1 | LOXIMVI-signal1 | B2 | |
| Vemurafenib | LOXIMVI | 5.0000 | uM | 24 | hr | | p27 Kip1 | 487.55 | p-Rb(S807/S811) | 84.67 | p-ERK(T202/Y204) | 31.63 | 2 | LOXIMVI-signal1 | C2 | |
| Vemurafenib | COLO 858 | JNK-IN-8 | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 416.80 | p-Rb(S807/S811) | 107.61 | p-S6(S235/S236 | 19.41 | 1 | Colo858-signal2 | F2 |
| Vemurafenib | COLO 858 | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 259.21 | p-Rb(S807/S811) | 68.62 | p-S6(S235/S236 | 16.39 | 2 | Colo858-signal2 | C2 | |
| JNK-IN-8 | COLO 858 | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 370.03 | p-Rb(S807/S811) | 260.57 | p-S6(S235/S236 | 614.60 | 2 | Colo858-signal2 | E2 | |
| Vemurafenib | COLO 858 | JNK-IN-8 | 5.0000 | uM | 24 | hr | | p27 Kip1 | 1646.10 | p-Rb(S807/S811) | 108.76 | p-ERK(T202/Y204) | 29.73 | 1 | Colo858-signal1 | F2 |
| Vemurafenib | COLO 858 | 5.0000 | uM | 24 | hr | | Ki-67 | 801.46 | p-Rb(S807/S811) | 44.01 | p-cJun(S73) | 45.24 | 1 | Colo858-signal3 | B2 | |
| Vemurafenib | COLO 858 | JNK-IN-8 | 5.0000 | uM | 24 | hr | | p27 Kip1 | 1526.80 | p-Rb(S807/S811) | 101.09 | p-ERK(T202/Y204) | 29.24 | 2 | Colo858-signal1 | G2 |
| JNK-IN-8 | COLO 858 | 5.0000 | uM | 24 | hr | | p27 Kip1 | 839.15 | p-Rb(S807/S811) | 256.43 | p-ERK(T202/Y204) | 789.95 | 2 | Colo858-signal1 | E2 | |
| Vemurafenib | COLO 858 | 5.0000 | uM | 24 | hr | | p27 Kip1 | 1100.60 | p-Rb(S807/S811) | 49.89 | p-ERK(T202/Y204) | 9.41 | 2 | Colo858-signal1 | C2 | |
| JNK-IN-8 | COLO 858 | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 315.08 | p-Rb(S807/S811) | 253.54 | p-S6(S235/S236 | 620.64 | 1 | Colo858-signal2 | D2 | |
| JNK-IN-8 | COLO 858 | 5.0000 | uM | 24 | hr | | p27 Kip1 | 738.89 | p-Rb(S807/S811) | 249.29 | p-ERK(T202/Y204) | 766.04 | 1 | Colo858-signal1 | D2 | |
| Vemurafenib | COLO 858 | 5.0000 | uM | 24 | hr | | p27 Kip1 | 1101.80 | p-Rb(S807/S811) | 48.19 | p-ERK(T202/Y204) | 9.79 | 1 | Colo858-signal1 | B2 | |
| Vemurafenib | LOXIMVI | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 210.99 | p-Rb(S807/S811) | 80.53 | p-S6(S235/S236 | 102.92 | 1 | LOXIMVI-signal2 | B2 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 5.0000 | uM | 24 | hr | | p27 Kip1 | 609.38 | p-Rb(S807/S811) | 99.97 | p-ERK(T202/Y204) | 70.02 | 2 | LOXIMVI-signal1 | G2 |