Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
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| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Vemurafenib | LOXIMVI | 0.0033 | uM | 24 | hr | | p27 Kip1 | 339.87 | p-Rb(S807/S811) | 207.46 | p-ERK(T202/Y204) | 188.18 | 1 | LOXIMVI-signal1 | B10 | |
| Vemurafenib | LOXIMVI | 0.0033 | uM | 24 | hr | | Ki-67 | 2929.90 | p-Rb(S807/S811) | 202.41 | p-cJun(S73) | 113.58 | 1 | LOXIMVI-signal3 | B10 | |
| Vemurafenib | WM-115 | 0.0033 | uM | 24 | hr | | Ki-67 | 734.74 | p-Rb(S807/S811) | 72.53 | p-cJun(S73) | 47.91 | 1 | WM115-signal3 | B10 | |
| Vemurafenib | LOXIMVI | 0.0033 | uM | 24 | hr | | p-Histone H3(S10) | 290.41 | p-Rb(S807/S811) | 222.56 | p-S6(S235/S236 | 435.26 | 1 | LOXIMVI-signal2 | B10 | |
| Vemurafenib | COLO 858 | 0.0033 | uM | 24 | hr | | p27 Kip1 | 452.40 | p-Rb(S807/S811) | 159.51 | p-ERK(T202/Y204) | 579.26 | 1 | Colo858-signal1 | B10 | |
| Vemurafenib | WM-115 | 0.0033 | uM | 24 | hr | | p-Histone H3(S10) | 218.20 | p-Rb(S807/S811) | 70.93 | p-S6(S235/S236 | 195.97 | 1 | WM115-signal2 | B10 | |
| Vemurafenib | WM1552C | 0.0033 | uM | 24 | hr | | p-Histone H3(S10) | 226.97 | p-Rb(S807/S811) | 112.19 | p-S6(S235/S236 | 400.08 | 1 | WM1552C-signal2 | B10 | |
| Vemurafenib | WM-115 | 0.0033 | uM | 24 | hr | | p27 Kip1 | 228.00 | p-Rb(S807/S811) | 72.02 | p-ERK(T202/Y204) | 575.75 | 1 | WM115-signal1 | B10 | |
| Vemurafenib | WM1552C | 0.0033 | uM | 24 | hr | | p27 Kip1 | 246.89 | p-Rb(S807/S811) | 106.93 | p-ERK(T202/Y204) | 243.94 | 1 | WM1552C-signal1 | B10 | |
| Vemurafenib | COLO 858 | 0.0033 | uM | 24 | hr | | p-Histone H3(S10) | 326.58 | p-Rb(S807/S811) | 162.30 | p-S6(S235/S236 | 402.84 | 1 | Colo858-signal2 | B10 | |
| Vemurafenib | WM1552C | 0.0033 | uM | 24 | hr | | Ki-67 | 1556.10 | p-Rb(S807/S811) | 107.86 | p-cJun(S73) | 143.87 | 1 | WM1552C-signal3 | B10 | |
| Vemurafenib | COLO 858 | 0.0033 | uM | 24 | hr | | Ki-67 | 2154.80 | p-Rb(S807/S811) | 145.55 | p-cJun(S73) | 28.64 | 1 | Colo858-signal3 | B10 | |
| Vemurafenib | COLO 858 | 0.0000 | uM | 24 | hr | | Ki-67 | 2209.00 | p-Rb(S807/S811) | 151.07 | p-cJun(S73) | 28.62 | 1 | Colo858-signal3 | B11 | |
| Vemurafenib | WM1552C | 0.0000 | uM | 24 | hr | | Ki-67 | 1693.60 | p-Rb(S807/S811) | 110.42 | p-cJun(S73) | 138.63 | 1 | WM1552C-signal3 | B11 | |
| Vemurafenib | WM1552C | 0.0000 | uM | 24 | hr | | p-Histone H3(S10) | 221.69 | p-Rb(S807/S811) | 117.69 | p-S6(S235/S236 | 423.70 | 1 | WM1552C-signal2 | B11 | |
| Vemurafenib | WM-115 | 0.0000 | uM | 24 | hr | | Ki-67 | 738.43 | p-Rb(S807/S811) | 73.77 | p-cJun(S73) | 49.75 | 1 | WM115-signal3 | B11 | |
| Vemurafenib | LOXIMVI | 0.0000 | uM | 24 | hr | | Ki-67 | 3070.70 | p-Rb(S807/S811) | 206.62 | p-cJun(S73) | 122.85 | 1 | LOXIMVI-signal3 | B11 | |
| Vemurafenib | LOXIMVI | 0.0000 | uM | 24 | hr | | p27 Kip1 | 346.49 | p-Rb(S807/S811) | 215.22 | p-ERK(T202/Y204) | 215.11 | 1 | LOXIMVI-signal1 | B11 | |
| Vemurafenib | LOXIMVI | 0.0000 | uM | 24 | hr | | p-Histone H3(S10) | 304.49 | p-Rb(S807/S811) | 234.76 | p-S6(S235/S236 | 449.34 | 1 | LOXIMVI-signal2 | B11 | |
| Vemurafenib | WM-115 | 0.0000 | uM | 24 | hr | | p27 Kip1 | 242.95 | p-Rb(S807/S811) | 73.13 | p-ERK(T202/Y204) | 635.44 | 1 | WM115-signal1 | B11 | |
| Vemurafenib | COLO 858 | 0.0000 | uM | 24 | hr | | p27 Kip1 | 479.59 | p-Rb(S807/S811) | 159.95 | p-ERK(T202/Y204) | 654.45 | 1 | Colo858-signal1 | B11 | |
| Vemurafenib | COLO 858 | 0.0000 | uM | 24 | hr | | p-Histone H3(S10) | 358.86 | p-Rb(S807/S811) | 155.99 | p-S6(S235/S236 | 377.96 | 1 | Colo858-signal2 | B11 | |
| Vemurafenib | WM-115 | 0.0000 | uM | 24 | hr | | p-Histone H3(S10) | 230.02 | p-Rb(S807/S811) | 70.34 | p-S6(S235/S236 | 211.00 | 1 | WM115-signal2 | B11 | |
| Vemurafenib | WM1552C | 0.0000 | uM | 24 | hr | | p27 Kip1 | 243.77 | p-Rb(S807/S811) | 107.70 | p-ERK(T202/Y204) | 254.74 | 1 | WM1552C-signal1 | B11 | |
| Vemurafenib | WM-115 | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 231.89 | p-Rb(S807/S811) | 45.77 | p-S6(S235/S236 | 35.99 | 1 | WM115-signal2 | B2 |