Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset - HMS LINCS Database

Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule	Cell Line	Drug Concentration	Conc Unit	Time Point	Time Point Unit	Replicate	Scanner/Software	pMEK(S217/221)	Normalized Median pMEK(S217/221)	pERK(T202/Y204)	Normalized Median pERK(T202/Y204)	p-p90RSK(S380)	Normalized Median p-p90RSK(S380)	p-p90RSK(T573)	Normalized Median p-p90RSK(T573)	p-AKT(T308)	Normalized Median p-AKT(T308)	p-AKT(S473)	Normalized Median p-AKT(S473)	p-mTOR(S2448)	Normalized Median p-mTOR(S2448)	p-p70S6K(T421/S424)	Normalized Median p-p70S6K(T421/S424)	p-p70S6K(T389)	Normalized Median p-p70S6K(T389)	p-S6(S235/236)	Normalized Median p-S6(S235/236)	p-AMPK(T172)	Normalized Median p-AMPK(T172)	p-JNK(T183/Y185)	Normalized Median p-JNK(T183/Y185)	Total c-Jun	Normalized Median Total c-Jun	p-cJun(S63)	Normalized Median p-cJun(S63)	p-P38(T180/Y182)	Normalized Median p-P38(T180/Y182)	p-HSP27(S82)	Normalized Median p-HSP27(S82)	p-NFKB(S536)	Normalized Median p-NFKB(S536)	Bim	Normalized Median Bim	cPARP	Normalized Median cPARP	p-Histone H3(S10)	Normalized Median p-Histone H3(S10)	p27 Kip1	Normalized Median p27 Kip1
	C32	0.0000	uM	48	hr	6	InnoScan/Mapix
	C32	0.0000	uM	48	hr	6	InnoScan/Mapix
	C32	0.0000	uM	48	hr	6	InnoScan/Mapix
	C32	0.0000	uM	48	hr	6	InnoScan/Mapix
	C32	0.0000	uM	48	hr	7	InnoScan/Mapix	0.2710		5.2632				0.0450		0.1035		0.5076				0.1828				0.2041		0.1043		0.3165		0.1045				0.0857				0.1479		0.2717		0.0883		0.5587		0.0913
	C32	0.0000	uM	48	hr	7	InnoScan/Mapix	0.2825		5.8824				0.0331		0.1081		0.4762				0.1767				0.1610		0.0908		0.3704		0.1044				0.1073				0.1431		0.2404		0.0678		0.5465		0.0950
	C32	0.0000	uM	48	hr	7	InnoScan/Mapix	0.2488		5.5556				0.0361		0.0960		0.4425				0.1724				0.1721		0.0758		0.3058		0.0990				0.0809				0.1629		0.2004		0.0698		0.5208		0.0889
	C32	0.0000	uM	48	hr	7	InnoScan/Mapix	0.2857		5.0000				0.0527		0.1049		0.4348				0.1764				0.1852		0.0810		0.3226		0.1218				0.1048				0.1304		0.2101		0.0661		0.5495		0.1007
	C32	0.0000	uM	48	hr	7	InnoScan/Mapix
	C32	0.0000	uM	48	hr	7	InnoScan/Mapix
	C32	0.0000	uM	48	hr	7	InnoScan/Mapix
	C32	0.0000	uM	48	hr	7	InnoScan/Mapix
	C32	0.0000	uM	48	hr	8	InnoScan/Mapix	0.3049		4.7619				0.0470		0.1110		0.4425				0.1650				0.1818		0.0810		0.2525		0.1242				0.0885				0.1799		0.2247		0.0786		0.4405		0.1093
	C32	0.0000	uM	48	hr	8	InnoScan/Mapix	0.3279		5.2632				0.0376		0.0883		0.4016				0.1603				0.1610		0.0782		0.2778		0.1217				0.1037				0.1724		0.2066		0.0739		0.4386		0.0842
	C32	0.0000	uM	48	hr	8	InnoScan/Mapix	0.2770		4.5455				0.0350		0.0857		0.3704				0.1553				0.1739		0.0617		0.2208		0.1131				0.0805				0.1592		0.1387		0.0591		0.4049		0.0793
	C32	0.0000	uM	48	hr	8	InnoScan/Mapix	0.3135		4.5455				0.0477		0.0899		0.3922				0.1667				0.2024		0.0657		0.2083		0.1229				0.0997				0.1477		0.1570		0.0654		0.4149		0.0737
	C32	0.0000	uM	48	hr	8	InnoScan/Mapix
	C32	0.0000	uM	48	hr	8	InnoScan/Mapix
	C32	0.0000	uM	48	hr	8	InnoScan/Mapix
	C32	0.0000	uM	48	hr	8	InnoScan/Mapix
SB590885	COLO 858	0.0032	uM	1	hr	1	InnoScan/Mapix	0.0264	-0.8780	0.7042	0.5986	0.0031	-0.2972	0.0031	-0.0711	0.0142	0.7701	0.0105	0.4833	0.0074	-0.2291	0.0142	-0.1401	0.0445	-0.3558	0.6623	0.5164	0.0442	-0.2721	0.0028	-0.0502	0.0243	0.5396	0.0093	0.2918	0.0288	0.4736	0.0046	-0.1308	0.0923	0.4336	0.0188	0.9084	0.0161	0.8352	0.1681	0.2778	0.0136	-0.1567
SB590885	COLO 858	0.0032	uM	1	hr	2	InnoScan/Mapix	0.0275	-0.8780	0.8621	0.5986	0.0033	-0.2972	0.0036	-0.0711	0.0150	0.7701	0.0124	0.4833	0.0089	-0.2291	0.0136	-0.1401	0.0459	-0.3558	0.6173	0.5164	0.0504	-0.2721	0.0034	-0.0502	0.0252	0.5396	0.0109	0.2918	0.0320	0.4736	0.0049	-0.1308	0.0943	0.4336	0.0234	0.9084	0.0120	0.8352	0.1736	0.2778	0.0181	-0.1567
SB590885	COLO 858	0.0032	uM	1	hr	3	InnoScan/Mapix	0.0268	-0.8780	0.5319	0.5986		-0.2972		-0.0711	0.0107	0.7701	0.0087	0.4833	0.0094	-0.2291	0.0164	-0.1401	0.0546	-0.3558	0.4505	0.5164	0.0521	-0.2721	0.0042	-0.0502	0.0212	0.5396	0.0110	0.2918	0.0234	0.4736	0.0073	-0.1308	0.0606	0.4336	0.0213	0.9084	0.0150	0.8352	0.0937	0.2778	0.0188	-0.1567
SB590885	COLO 858	0.0032	uM	1	hr	4	InnoScan/Mapix	0.0291	-0.8780	0.8475	0.5986	0.0036	-0.2972	0.0033	-0.0711	0.0122	0.7701	0.0112	0.4833	0.0077	-0.2291	0.0155	-0.1401	0.0587	-0.3558	0.5236	0.5164	0.0500	-0.2721	0.0029	-0.0502	0.0262	0.5396	0.0085	0.2918	0.0351	0.4736	0.0059	-0.1308	0.0782	0.4336	0.0164	0.9084	0.0115	0.8352	0.1232	0.2778	0.0145	-0.1567
SB590885	COLO 858	0.0032	uM	1	hr	5	InnoScan/Mapix	0.0249	-0.8780	0.6667	0.5986	0.0044	-0.2972		-0.0711	0.0086	0.7701	0.0088	0.4833	0.0064	-0.2291	0.0112	-0.1401	0.0411	-0.3558	0.5650	0.5164	0.0484	-0.2721	0.0025	-0.0502	0.0230	0.5396	0.0086	0.2918	0.0283	0.4736	0.0054	-0.1308	0.0892	0.4336	0.0121	0.9084	0.0127	0.8352	0.1095	0.2778	0.0127	-0.1567

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HMS LINCS Project

Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)