Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
C32 0.0000
uM
 48
hr
 3
InnoScan/Mapix
 0.3311 5.2632 0.0678 0.1209 0.4878 0.1208 0.2075 0.0944 0.3718 0.1504 0.1192 0.1484 0.2959 0.0790 0.5376 0.1274
C32 0.0000
uM
 48
hr
 3
InnoScan/Mapix
C32 0.0000
uM
 48
hr
 3
InnoScan/Mapix
C32 0.0000
uM
 48
hr
 3
InnoScan/Mapix
C32 0.0000
uM
 48
hr
 3
InnoScan/Mapix
C32 0.0000
uM
 48
hr
 4
InnoScan/Mapix
 0.3300 5.5556 0.0476 0.1272 0.5236 0.1901 0.1908 0.1094 0.1942 0.1374 0.1055 0.1869 0.2793 0.0906 0.5263 0.1572
C32 0.0000
uM
 48
hr
 4
InnoScan/Mapix
 0.3584 5.8824 0.0527 0.1178 0.5102 0.1745 0.1739 0.1127 0.3030 0.1238 0.1252 0.1890 0.3115 0.0843 0.5128 0.1120
C32 0.0000
uM
 48
hr
 4
InnoScan/Mapix
 0.3077 5.2632 0.0319 0.1504 0.4484 0.1789 0.1919 0.0777 0.2710 0.1395 0.0961 0.1770 0.2110 0.0699 0.4630 0.0951
C32 0.0000
uM
 48
hr
 4
InnoScan/Mapix
 0.3663 5.2632 0.0621 0.1064 0.4673 0.1931 0.2119 0.0948 0.2703 0.1462 0.1139 0.1618 0.2639 0.0812 0.4762 0.1212
C32 0.0000
uM
 48
hr
 4
InnoScan/Mapix
C32 0.0000
uM
 48
hr
 4
InnoScan/Mapix
C32 0.0000
uM
 48
hr
 4
InnoScan/Mapix
C32 0.0000
uM
 48
hr
 4
InnoScan/Mapix
C32 0.0000
uM
 48
hr
 5
InnoScan/Mapix
 0.3663 5.5556 0.0653 0.1274 0.5525 0.2169 0.2740 0.1142 0.3968 0.1495 0.1129 0.1695 0.3115 0.0856 0.5814 0.1106
C32 0.0000
uM
 48
hr
 5
InnoScan/Mapix
 0.3759 5.5556 0.0849 0.1342 0.5208 0.2222 0.2457 0.1171 0.4149 0.1590 0.1441 0.2114 0.3922 0.0991 0.5952 0.1379
C32 0.0000
uM
 48
hr
 5
InnoScan/Mapix
 0.3788 5.2632 0.0603 0.1110 0.4902 0.1972 0.2457 0.0896 0.3846 0.1454 0.1055 0.1695 0.2519 0.0441 0.4831 0.1205
C32 0.0000
uM
 48
hr
 5
InnoScan/Mapix
 0.3690 5.0000 0.0794 0.1229 0.4762 0.1934 0.2364 0.1013 0.4000 0.1383 0.1116 0.1894 0.2584 0.0927 0.5128 0.1256
C32 0.0000
uM
 48
hr
 5
InnoScan/Mapix
C32 0.0000
uM
 48
hr
 5
InnoScan/Mapix
C32 0.0000
uM
 48
hr
 5
InnoScan/Mapix
C32 0.0000
uM
 48
hr
 5
InnoScan/Mapix
C32 0.0000
uM
 48
hr
 6
InnoScan/Mapix
 0.3333 5.2632 0.0494 0.1177 0.5405 0.1873 0.2288 0.0967 0.4065 0.1138 0.0940 0.1527 0.2924 0.0623 0.5155 0.1049
C32 0.0000
uM
 48
hr
 6
InnoScan/Mapix
 0.3290 6.2500 0.0453 0.1099 0.5102 0.1988 0.1639 0.0979 0.4098 0.1057 0.1186 0.1742 0.2632 0.0792 0.5025 0.1042
C32 0.0000
uM
 48
hr
 6
InnoScan/Mapix
 0.3497 5.2632 0.0437 0.1013 0.4546 0.1815 0.2008 0.0828 0.3636 0.1171 0.0962 0.1764 0.2604 0.0814 0.4717 0.0973
C32 0.0000
uM
 48
hr
 6
InnoScan/Mapix
 0.3290 5.5556 0.0582 0.1131 0.4546 0.1898 0.1852 0.0867 0.3584 0.1179 0.1100 0.1414 0.2370 0.0695 0.4695 0.1124