Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
WM-115 0.0000
uM
 48
hr
 4
Odyssey/Microvigene
 0.0625 0.3300 0.0114 0.0701 0.0354 0.0870 0.0178 0.0215 0.0381 0.0326 0.0145 0.0232 0.0280 0.0791
WM-115 0.0000
uM
 48
hr
 4
Odyssey/Microvigene
 0.0567 0.3704 0.0106 0.0118 0.0973 0.0381 0.0955 0.0191 0.0269 0.0324 0.0332 0.0178 0.0213 0.0296 0.0213
WM-115 0.0000
uM
 48
hr
 4
Odyssey/Microvigene
 0.0575 0.3401 0.0121 0.0117 0.0928 0.0370 0.0988 0.0165 0.0254 0.0337 0.0342 0.0189 0.0225 0.0293 0.0771 0.0203
WM-115 0.0000
uM
 48
hr
 4
Odyssey/Microvigene
 0.0512 0.3953 0.0128 0.0133 0.1167 0.1164 0.0229 0.0283 0.0352 0.0405 0.0222 0.0270 0.0289 0.0810 0.0246
WM-115 0.0000
uM
 48
hr
 4
Odyssey/Microvigene
 0.0543 0.3378 0.0086 0.0098 0.0627 0.0324 0.0750 0.0170 0.0246 0.0282 0.0323 0.0162 0.0210 0.0245 0.0829 0.0195
WM-115 0.0000
uM
 48
hr
 4
Odyssey/Microvigene
 0.0504 0.3891 0.0101 0.0112 0.0833 0.0393 0.0852 0.0209 0.0263 0.0305 0.0386 0.0178 0.0238 0.0279 0.0916 0.0220
WM-115 0.0000
uM
 48
hr
 4
Odyssey/Microvigene
 0.0641 0.3268 0.0082 0.0084 0.0629 0.0309 0.0625 0.0184 0.0204 0.0301 0.0312 0.0160 0.0311 0.0864 0.0210
WM-115 0.0000
uM
 48
hr
 4
Odyssey/Microvigene
 0.0630 0.3891 0.0107 0.0086 0.0826 0.0462 0.0874 0.0207 0.0252 0.0355 0.0328 0.0191 0.0189 0.0332 0.0822 0.0247
WM-115 0.0000
uM
 48
hr
 5
Odyssey/Microvigene
 0.0630 0.3831 0.0082 0.0086 0.0783 0.0363 0.1009 0.0197 0.0237 0.0304 0.0292 0.0201 0.0259 0.0284 0.0204
WM-115 0.0000
uM
 48
hr
 5
Odyssey/Microvigene
 0.0699 0.4444 0.0087 0.0097 0.0727 0.0372 0.1045 0.0184 0.0279 0.0243 0.0334 0.0198 0.0192 0.0258 0.0881
WM-115 0.0000
uM
 48
hr
 5
Odyssey/Microvigene
 0.0630 0.3636 0.0079 0.0093 0.0770 0.0425 0.0938 0.0173 0.0228 0.0376 0.0316 0.0148 0.0239 0.0349 0.0852 0.0204
WM-115 0.0000
uM
 48
hr
 5
Odyssey/Microvigene
 0.0747 0.4348 0.0101 0.0108 0.0853 0.0434 0.1091 0.0206 0.0252 0.0350 0.0346 0.0165 0.0232 0.0334 0.0846 0.0204
WM-115 0.0000
uM
 48
hr
 5
Odyssey/Microvigene
 0.0646 0.3937 0.0084 0.0099 0.0815 0.0349 0.0997 0.0164 0.0261 0.0347 0.0302 0.0168 0.0270 0.0340 0.0879 0.0186
WM-115 0.0000
uM
 48
hr
 5
Odyssey/Microvigene
 0.0725 0.4608 0.0115 0.0098 0.0776 0.0471 0.1034 0.0199 0.0283 0.0299 0.0366 0.0181 0.0236 0.0314 0.0207
WM-115 0.0000
uM
 48
hr
 5
Odyssey/Microvigene
 0.0693 0.3345 0.0111 0.0178 0.0755 0.0409 0.0873 0.0157 0.0224 0.0334 0.0377 0.0155 0.0190 0.0317 0.0869 0.0192
WM-115 0.0000
uM
 48
hr
 5
Odyssey/Microvigene
 0.0745 0.4115 0.0101 0.0821 0.0376 0.0914 0.0181 0.0207 0.0339 0.0300 0.0146 0.0240 0.0296 0.0826 0.0212
WM-115 0.0000
uM
 48
hr
 6
Odyssey/Microvigene
 0.0705 0.3345 0.0080 0.0782 0.0369 0.0932 0.0174 0.0235 0.0314 0.0287 0.0232 0.0267 0.0327 0.0784
WM-115 0.0000
uM
 48
hr
 6
Odyssey/Microvigene
 0.0790 0.3597 0.0078 0.0104 0.0808 0.0310 0.1012 0.0146 0.0226 0.0280 0.0280 0.0144 0.0169 0.0313 0.0913
WM-115 0.0000
uM
 48
hr
 6
Odyssey/Microvigene
 0.0665 0.3030 0.0081 0.0136 0.0725 0.0361 0.0929 0.0155 0.0201 0.0345 0.0283 0.0152 0.0222 0.0324 0.0865 0.0191
WM-115 0.0000
uM
 48
hr
 6
Odyssey/Microvigene
 0.0703 0.3521 0.0111 0.0957 0.0406 0.1013 0.0186 0.0240 0.0344 0.0167 0.0240 0.0314 0.0908 0.0188
WM-115 0.0000
uM
 48
hr
 6
Odyssey/Microvigene
 0.0732 0.3236 0.0082 0.0087 0.0664 0.0340 0.0839 0.0163 0.0227 0.0322 0.0291 0.0283 0.0225 0.0357 0.0929 0.0187
WM-115 0.0000
uM
 48
hr
 6
Odyssey/Microvigene
 0.0714 0.3774 0.0090 0.0094 0.0732 0.0349 0.0903 0.0159 0.0231 0.0327 0.0297 0.0184 0.0204 0.0321 0.1094 0.0172
WM-115 0.0000
uM
 48
hr
 6
Odyssey/Microvigene
 0.0808 0.2976 0.0091 0.0083 0.0656 0.0330 0.0806 0.0135 0.0189 0.0334 0.0279 0.0133 0.0168 0.0368 0.1036 0.0166
WM-115 0.0000
uM
 48
hr
 6
Odyssey/Microvigene
 0.0739 0.3677 0.0099 0.0102 0.0792 0.0341 0.0839 0.0162 0.0203 0.0343 0.0299 0.0131 0.0200 0.0352 0.0911 0.0201
WM-115 0.0000
uM
 48
hr
 7
Odyssey/Microvigene
 0.0647 0.3030 0.0070 0.0077 0.0618 0.0310 0.0836 0.0167 0.0222 0.0293 0.0303 0.0223 0.0222 0.0300 0.0755 0.0264