Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
RVH-421 0.0000
uM
 24
hr
 7
Odyssey/Microvigene
 0.5208 1.1751 0.1980 0.3135 0.2416 0.6711 0.4425 0.3906 0.9091 0.2564 0.5780 0.2770 0.2653 0.3106 0.2088 0.2809 0.2041 0.7576 0.2833
RVH-421 0.0000
uM
 24
hr
 7
Odyssey/Microvigene
 0.5587 1.0650 0.1927 0.3448 0.2342 0.6757 0.4274 0.3759 0.9346 0.2320 0.2725 0.2198 0.3155 0.2304 0.2494 0.1848 0.7874 0.3257
RVH-421 0.0000
uM
 24
hr
 7
Odyssey/Microvigene
 0.5556 1.0482 0.2000 0.3205 0.2387 0.6536 0.4255 0.4525 0.9709 0.2494 0.5556 0.2809 0.2398 0.3460 0.2123 0.2890 0.2012 0.8265 0.2865
RVH-421 0.0000
uM
 24
hr
 8
Odyssey/Microvigene
 0.5780 1.3423 0.2016 0.3040 0.2519 0.6289 0.4405 0.3413 0.2732 0.5587 0.3021 0.2564 0.4065 0.1992 0.3460 0.2212 0.7874 0.3096
RVH-421 0.0000
uM
 24
hr
 8
Odyssey/Microvigene
 0.5747 1.2107 0.2037 0.3012 0.2732 0.5814 0.4566 0.3484 0.9434 0.2681 0.6098 0.2604 0.2427 0.4310 0.2053 0.2899 0.2020 0.7692 0.2994
RVH-421 0.0000
uM
 24
hr
 8
Odyssey/Microvigene
 0.5848 1.1403 0.2033 0.3521 0.2174 0.6494 0.4566 0.3650 0.8850 0.2584 0.5495 0.2950 0.2310 0.3817 0.2041 0.3096 0.1855 0.7463 0.3155
RVH-421 0.0000
uM
 24
hr
 8
Odyssey/Microvigene
 1.3850 0.2092 0.3175 0.2525 0.6211 0.4566 0.4016 0.9434 0.2817 0.5952 0.3390 0.2571 0.4255 0.2033 0.2950 0.2299 0.8065 0.3195
RVH-421 0.0000
uM
 24
hr
 8
Odyssey/Microvigene
 0.5291 1.0823 0.1965 0.3247 0.2404 0.6579 0.4149 0.3876 0.9901 0.2439 0.5587 0.2950 0.2347 0.3401 0.2217 0.3257 0.1883 0.7246 0.3106
RVH-421 0.0000
uM
 24
hr
 8
Odyssey/Microvigene
 0.5650 1.1765 0.1901 0.2857 0.2558 0.6536 0.4367 0.3906 0.9009 0.2674 0.5917 0.2865 0.2500 0.3077 0.2232 0.2725 0.2101 0.7246 0.3086
RVH-421 0.0000
uM
 24
hr
 8
Odyssey/Microvigene
 0.5587 0.9804 0.3185 0.2155 0.6211 0.4132 0.3718 0.9259 0.2326 0.4505 0.2770 0.2227 0.3106 0.2128 0.2695 0.2037 0.7692 0.2985
RVH-421 0.0000
uM
 24
hr
 8
Odyssey/Microvigene
 0.5882 1.2121 0.1996 0.2865 0.2571 0.6250 0.4505 0.4049 0.9615 0.2475 0.4975 0.3268 0.2208 0.3356 0.1946 0.2604 0.2110 0.8065 0.3058
RVH-421 0.0000
uM
 48
hr
 1
Odyssey/Microvigene
 0.5348 1.1962 0.2212 0.3448 0.2519 0.7407 0.4975 0.3802 0.7519 0.2475 0.5291 0.3106 0.2725 0.3788 0.2247 0.3484 0.2445 0.7042 0.3185
RVH-421 0.0000
uM
 48
hr
 1
Odyssey/Microvigene
 0.5263 1.1891 0.2564 0.3472 0.2899 0.7246 0.4608 0.3891 0.7092 0.2625 0.5814 0.2967 0.2841 0.3891 0.2083 0.2976 0.2247 0.7299 0.3663
RVH-421 0.0000
uM
 48
hr
 1
Odyssey/Microvigene
 0.5076 1.1416 0.2232 0.3390 0.2398 0.7353 0.4525 0.3663 0.6993 0.2538 0.5714 0.3145 0.2604 0.3690 0.2208 0.3390 0.2062 0.6849 0.3401
RVH-421 0.0000
uM
 48
hr
 1
Odyssey/Microvigene
 0.5405 1.1723 0.2222 0.3257 0.2660 0.7042 0.5128 0.3906 0.7353 0.2625 0.6536 0.3460 0.2632 0.4032 0.2110 0.3268 0.2310 0.7092 0.3425
RVH-421 0.0000
uM
 48
hr
 1
Odyssey/Microvigene
 0.5435 1.0953 0.2188 0.3774 0.2451 0.7042 0.4785 0.3333 0.7246 0.2577 0.5376 0.3058 0.2755 0.3623 0.2232 0.4032 0.2353 0.7194 0.3175
RVH-421 0.0000
uM
 48
hr
 1
Odyssey/Microvigene
 0.5291 1.1710 0.2597 0.3704 0.2584 0.6897 0.3636 0.7194 0.2667 0.6061 0.3322 0.2755 0.3774 0.2252 0.3677 0.2439 0.7463 0.3290
RVH-421 0.0000
uM
 48
hr
 1
Odyssey/Microvigene
 0.5465 1.1236 0.2165 0.3817 0.7092 0.4651 0.3534 0.7353 0.2571 0.5814 0.3436 0.2519 0.3460 0.2416 0.3086 0.2049 0.7874 0.3300
RVH-421 0.0000
uM
 48
hr
 1
Odyssey/Microvigene
 1.1025 0.2387 0.3300 0.2538 0.6944 0.4831 0.4032 0.7692 0.2632 0.6135 0.3345 0.2646 0.3650 0.2037 0.3165 0.2381 0.7937 0.3425
RVH-421 0.0000
uM
 48
hr
 2
Odyssey/Microvigene
 0.5525 1.1148 0.1927 0.3290 0.2020 0.7299 0.4367 0.3300 0.6897 0.2342 0.4608 0.2500 0.2320 0.3333 0.2049 0.2985 0.1890 0.6623 0.3175
RVH-421 0.0000
uM
 48
hr
 2
Odyssey/Microvigene
 0.5128 1.0858 0.2066 0.3215 0.2353 0.7407 0.4608 0.2967 0.7463 0.2208 0.5291 0.2525 0.2439 0.3290 0.2169 0.2786 0.1946 0.6623 0.2874
RVH-421 0.0000
uM
 48
hr
 2
Odyssey/Microvigene
 0.5208 1.1186 0.1901 0.3135 0.2193 0.7092 0.4505 0.3413 0.7246 0.2299 0.4902 0.2849 0.2283 0.3333 0.1953 0.2941 0.2088 0.7299 0.3068
RVH-421 0.0000
uM
 48
hr
 2
Odyssey/Microvigene
 0.5102 1.1062 0.2028 0.2882 0.2558 0.6897 0.4878 0.3597 0.7463 0.2364 0.6024 0.2985 0.2513 0.3497 0.2151 0.3106 0.2165 0.7634 0.3236
RVH-421 0.0000
uM
 48
hr
 2
Odyssey/Microvigene
 0.5376 1.1123 0.2137 0.3049 0.2070 0.6623 0.4444 0.3817 0.6944 0.2500 0.4630 0.2681 0.2242 0.3247 0.1972 0.3165 0.2037 0.6803 0.3226
RVH-421 0.0000
uM
 48
hr
 2
Odyssey/Microvigene
 0.4902 1.1186 0.1992 0.3205 0.2667 0.6803 0.4274 0.3345 0.7194 0.2347 0.5747 0.2924 0.2392 0.3367 0.2037 0.3106 0.2257 0.6944 0.2994