Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
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| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| Gefitinib | HCC1954 | 1.0 | nM | 6 | hr | 274.52 | 71.74 | p-Akt | 25.25 | |
| Gefitinib | HCC1954 | 1.0 | nM | 24 | hr | 286.75 | 116.95 | p-Akt | 76.49 | |
| Torkinib | HCC1954 | 0.1 | nM | 6 | hr | 184.63 | 45.52 | p-Akt | 20.62 | |
| Torkinib | HCC1954 | 0.1 | nM | 24 | hr | 300.36 | 112.32 | p-Akt | 67.70 | |
| GSK1059615 | HCC1954 | 1.0 | nM | 6 | hr | 486.59 | 98.07 | p-Akt | 57.03 | |
| GSK1059615 | HCC1954 | 1.0 | nM | 24 | hr | 285.61 | 106.37 | p-Akt | 66.10 | |
| MK2206 | HCC1954 | 1.0 | nM | 6 | hr | 332.09 | 92.24 | p-Akt | 50.09 | |
| MK2206 | HCC1954 | 1.0 | nM | 24 | hr | 305.60 | 113.90 | p-Akt | 65.09 | |
| Triciribine | HCC1954 | 1.0 | nM | 6 | hr | 251.09 | 80.77 | p-Akt | 44.35 | |
| Triciribine | HCC1954 | 1.0 | nM | 24 | hr | 324.38 | 110.64 | p-Akt | 62.61 | |
| Dactolisib | HCC1954 | 1.0 | nM | 6 | hr | 188.42 | 59.42 | p-Akt | 32.78 | |
| Dactolisib | HCC1954 | 1.0 | nM | 24 | hr | 289.99 | 106.28 | p-Akt | 72.70 | |
| Gefitinib | MCF 10A | 1.0 | nM | 6 | hr | 406.40 | 163.48 | p-Akt | 45.06 | |
| Gefitinib | MCF 10A | 1.0 | nM | 24 | hr | 305.19 | 131.65 | p-Akt | 50.78 | |
| Torkinib | MCF 10A | 0.1 | nM | 6 | hr | 483.02 | 168.09 | p-Akt | 48.88 | |
| Torkinib | MCF 10A | 0.1 | nM | 24 | hr | 357.13 | 139.14 | p-Akt | 46.69 | |
| GSK1059615 | MCF 10A | 1.0 | nM | 6 | hr | 465.82 | 171.96 | p-Akt | 51.86 | |
| GSK1059615 | MCF 10A | 1.0 | nM | 24 | hr | 313.26 | 123.02 | p-Akt | 39.34 | |
| MK2206 | MCF 10A | 1.0 | nM | 6 | hr | 418.20 | 151.41 | p-Akt | 45.12 | |
| MK2206 | MCF 10A | 1.0 | nM | 24 | hr | 299.48 | 135.15 | p-Akt | 40.84 | |
| Triciribine | MCF 10A | 1.0 | nM | 6 | hr | 419.80 | 152.81 | p-Akt | 45.30 | |
| Triciribine | MCF 10A | 1.0 | nM | 24 | hr | 264.10 | 123.14 | p-Akt | 36.33 | |
| Dactolisib | MCF 10A | 1.0 | nM | 6 | hr | 436.43 | 157.03 | p-Akt | 43.37 | |
| Dactolisib | MCF 10A | 1.0 | nM | 24 | hr | 262.72 | 128.84 | p-Akt | 34.85 | |
| Gefitinib | T47D | 1.0 | nM | 6 | hr | 186.25 | 95.12 | p-Akt | 100.55 | |