Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
Download: Excel (XLSX), Comma-separated (CSV)
| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| Dactolisib | MCF 10A | 0 (control) | 24 | hr | 343.08 | 120.76 | p-Akt | 47.16 | | |
| Gefitinib | T47D | 0 (control) | 6 | hr | 187.71 | 94.01 | p-Akt | 103.59 | | |
| Gefitinib | T47D | 0 (control) | 24 | hr | 170.59 | 100.50 | p-Akt | 77.77 | | |
| Torkinib | T47D | 0 (control) | 6 | hr | 175.86 | 85.25 | p-Akt | 102.65 | | |
| Torkinib | T47D | 0 (control) | 24 | hr | 159.62 | 95.25 | p-Akt | 70.79 | | |
| GSK1059615 | T47D | 0 (control) | 6 | hr | 156.94 | 85.75 | p-Akt | 89.93 | | |
| GSK1059615 | T47D | 0 (control) | 24 | hr | 157.54 | 89.78 | p-Akt | 65.53 | | |
| MK2206 | T47D | 0 (control) | 6 | hr | 93.47 | 32.94 | p-Akt | 14.46 | | |
| MK2206 | T47D | 0 (control) | 24 | hr | 168.73 | 94.72 | p-Akt | 64.26 | | |
| Triciribine | T47D | 0 (control) | 6 | hr | 98.11 | 30.56 | p-Akt | 14.84 | | |
| Triciribine | T47D | 0 (control) | 24 | hr | 176.48 | 88.36 | p-Akt | 66.59 | | |
| Dactolisib | T47D | 0 (control) | 6 | hr | 93.59 | 40.79 | p-Akt | 16.04 | | |
| Dactolisib | T47D | 0 (control) | 24 | hr | 164.85 | 91.26 | p-Akt | 58.26 | | |
| Gefitinib | AU565 | 1.0 | nM | 6 | hr | 329.62 | 68.74 | p-Akt | 34.37 | |
| Gefitinib | AU565 | 1.0 | nM | 24 | hr | 594.52 | 125.03 | p-Akt | 142.48 | |
| Torkinib | AU565 | 0.1 | nM | 6 | hr | 249.91 | 55.55 | p-Akt | 24.44 | |
| Torkinib | AU565 | 0.1 | nM | 24 | hr | 513.30 | 118.22 | p-Akt | 83.59 | |
| GSK1059615 | AU565 | 1.0 | nM | 6 | hr | 401.42 | 90.96 | p-Akt | 45.19 | |
| GSK1059615 | AU565 | 1.0 | nM | 24 | hr | 486.86 | 118.09 | p-Akt | 84.54 | |
| MK2206 | AU565 | 1.0 | nM | 6 | hr | 205.49 | 54.71 | p-Akt | 18.44 | |
| MK2206 | AU565 | 1.0 | nM | 24 | hr | 423.26 | 118.26 | p-Akt | 69.90 | |
| Triciribine | AU565 | 1.0 | nM | 6 | hr | 382.75 | 90.73 | p-Akt | 30.89 | |
| Triciribine | AU565 | 1.0 | nM | 24 | hr | 545.03 | 125.22 | p-Akt | 105.22 | |
| Dactolisib | AU565 | 1.0 | nM | 6 | hr | 211.05 | 49.08 | p-Akt | 19.76 | |
| Dactolisib | AU565 | 1.0 | nM | 24 | hr | 527.76 | 112.13 | p-Akt | 112.96 | |