Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
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| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| MK2206 | AU565 | 316.0 | nM | 24 | hr | 342.39 | 86.36 | p-4EBP1 | 122.77 | |
| Triciribine | AU565 | 316.0 | nM | 6 | hr | 374.37 | 86.05 | p-4EBP1 | 118.53 | |
| Triciribine | AU565 | 316.0 | nM | 24 | hr | 499.67 | 91.10 | p-4EBP1 | 143.95 | |
| Dactolisib | AU565 | 316.0 | nM | 6 | hr | 328.87 | 65.51 | p-4EBP1 | 9.30 | |
| Dactolisib | AU565 | 316.0 | nM | 24 | hr | 438.38 | 64.12 | p-4EBP1 | 13.69 | |
| Gefitinib | HCC1954 | 316.0 | nM | 6 | hr | 166.89 | 61.52 | p-4EBP1 | 80.63 | |
| Gefitinib | HCC1954 | 316.0 | nM | 24 | hr | 249.17 | 105.94 | p-4EBP1 | 124.32 | |
| Torkinib | HCC1954 | 133.0 | nM | 6 | hr | 178.50 | 38.79 | p-4EBP1 | 20.77 | |
| Torkinib | HCC1954 | 133.0 | nM | 24 | hr | 222.17 | 88.12 | p-4EBP1 | 55.50 | |
| GSK1059615 | HCC1954 | 316.0 | nM | 6 | hr | 326.62 | 79.30 | p-4EBP1 | 87.60 | |
| GSK1059615 | HCC1954 | 316.0 | nM | 24 | hr | 275.10 | 71.43 | p-4EBP1 | 75.04 | |
| MK2206 | HCC1954 | 316.0 | nM | 6 | hr | 189.81 | 54.51 | p-4EBP1 | 76.66 | |
| MK2206 | HCC1954 | 316.0 | nM | 24 | hr | 260.14 | 111.51 | p-4EBP1 | 138.64 | |
| Triciribine | HCC1954 | 316.0 | nM | 6 | hr | 260.64 | 86.03 | p-4EBP1 | 124.51 | |
| Triciribine | HCC1954 | 316.0 | nM | 24 | hr | 241.93 | 108.12 | p-4EBP1 | 118.63 | |
| Dactolisib | HCC1954 | 316.0 | nM | 6 | hr | 220.49 | 67.32 | p-4EBP1 | 10.28 | |
| Dactolisib | HCC1954 | 316.0 | nM | 24 | hr | 269.10 | 71.60 | p-4EBP1 | 15.75 | |
| Gefitinib | MCF 10A | 316.0 | nM | 6 | hr | 215.20 | 145.64 | p-4EBP1 | 265.74 | |
| Gefitinib | MCF 10A | 316.0 | nM | 24 | hr | 199.54 | 154.15 | p-4EBP1 | 149.46 | |
| Torkinib | MCF 10A | 133.0 | nM | 6 | hr | 498.92 | 152.17 | p-4EBP1 | 90.64 | |
| Torkinib | MCF 10A | 133.0 | nM | 24 | hr | 381.89 | 153.55 | p-4EBP1 | 79.52 | |
| GSK1059615 | MCF 10A | 316.0 | nM | 6 | hr | 271.28 | 151.18 | p-4EBP1 | 256.87 | |
| GSK1059615 | MCF 10A | 316.0 | nM | 24 | hr | 233.58 | 148.22 | p-4EBP1 | 134.07 | |
| MK2206 | MCF 10A | 316.0 | nM | 6 | hr | 374.57 | 150.53 | p-4EBP1 | 347.38 | |
| MK2206 | MCF 10A | 316.0 | nM | 24 | hr | 279.94 | 176.18 | p-4EBP1 | 195.13 | |