Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
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| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| Gefitinib | MCF 10A | 3.16 | nM | 6 | hr | 427.67 | 169.41 | p-4EBP1 | 298.33 | |
| Gefitinib | MCF 10A | 3.16 | nM | 24 | hr | 281.45 | 145.65 | p-4EBP1 | 262.63 | |
| Torkinib | MCF 10A | 0.422 | nM | 6 | hr | 460.02 | 163.94 | p-4EBP1 | 355.88 | |
| Torkinib | MCF 10A | 0.422 | nM | 24 | hr | 321.05 | 157.86 | p-4EBP1 | 262.23 | |
| GSK1059615 | MCF 10A | 3.16 | nM | 6 | hr | 413.32 | 170.40 | p-4EBP1 | 360.27 | |
| GSK1059615 | MCF 10A | 3.16 | nM | 24 | hr | 288.84 | 144.14 | p-4EBP1 | 262.85 | |
| MK2206 | MCF 10A | 3.16 | nM | 6 | hr | 390.81 | 157.12 | p-4EBP1 | 361.22 | |
| MK2206 | MCF 10A | 3.16 | nM | 24 | hr | 288.88 | 154.95 | p-4EBP1 | 271.07 | |
| Triciribine | MCF 10A | 3.16 | nM | 6 | hr | 393.04 | 157.90 | p-4EBP1 | 362.41 | |
| Triciribine | MCF 10A | 3.16 | nM | 24 | hr | 280.89 | 144.27 | p-4EBP1 | 264.66 | |
| Dactolisib | MCF 10A | 3.16 | nM | 6 | hr | 383.57 | 152.45 | p-4EBP1 | 353.00 | |
| Dactolisib | MCF 10A | 3.16 | nM | 24 | hr | 263.37 | 155.04 | p-4EBP1 | 239.83 | |
| Gefitinib | T47D | 3.16 | nM | 6 | hr | 186.66 | 102.68 | p-4EBP1 | 497.08 | |
| Gefitinib | T47D | 3.16 | nM | 24 | hr | 156.92 | 127.73 | p-4EBP1 | 422.23 | |
| Torkinib | T47D | 0.422 | nM | 6 | hr | 177.20 | 86.68 | p-4EBP1 | 519.62 | |
| Torkinib | T47D | 0.422 | nM | 24 | hr | 164.15 | 114.64 | p-4EBP1 | 409.39 | |
| GSK1059615 | T47D | 3.16 | nM | 6 | hr | 171.49 | 82.21 | p-4EBP1 | 477.19 | |
| GSK1059615 | T47D | 3.16 | nM | 24 | hr | 153.00 | 108.63 | p-4EBP1 | 405.03 | |
| MK2206 | T47D | 3.16 | nM | 6 | hr | 98.37 | 46.79 | p-4EBP1 | 109.35 | |
| MK2206 | T47D | 3.16 | nM | 24 | hr | 158.36 | 116.30 | p-4EBP1 | 402.92 | |
| Triciribine | T47D | 3.16 | nM | 6 | hr | 103.74 | 54.46 | p-4EBP1 | 177.89 | |
| Triciribine | T47D | 3.16 | nM | 24 | hr | 148.05 | 109.27 | p-4EBP1 | 380.75 | |
| Dactolisib | T47D | 3.16 | nM | 6 | hr | 95.07 | 41.72 | p-4EBP1 | 113.42 | |
| Dactolisib | T47D | 3.16 | nM | 24 | hr | 130.71 | 105.86 | p-4EBP1 | 330.99 | |
| Gefitinib | AU565 | 10.0 | nM | 6 | hr | 270.31 | 60.18 | p-4EBP1 | 67.03 | |