Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 1496 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 154.73 NGFR rabbit IgG(H+L) / donkey NGFR 148.32
A375-B1-rep1
H2
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 1462 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 154.89 NGFR rabbit IgG(H+L) / donkey NGFR 141.44
A375-B1-rep1
H3
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 1257 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 163.46 NGFR rabbit IgG(H+L) / donkey NGFR 150.95
A375-B1-rep1
H4
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 1235 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 168.84 NGFR rabbit IgG(H+L) / donkey NGFR 135.45
A375-B1-rep1
H5
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 1289 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 162.79 NGFR rabbit IgG(H+L) / donkey NGFR 134.80
A375-B1-rep1
H6
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 1386 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 162.16 NGFR rabbit IgG(H+L) / donkey NGFR 141.90
A375-B1-rep1
H7
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 1111 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 143.94 NGFR rabbit IgG(H+L) / donkey NGFR 127.79
A375-B1-rep1
H8
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 1314 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 167.73 NGFR rabbit IgG(H+L) / donkey NGFR 154.69
A375-B1-rep1
H9
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1697 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 193.98 NGFR rabbit IgG(H+L) / donkey NGFR 177.83
A375-B1-rep2
A10
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1490 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 206.53 NGFR rabbit IgG(H+L) / donkey NGFR 194.20
A375-B1-rep2
A11
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 2108 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 195.39 NGFR rabbit IgG(H+L) / donkey NGFR 201.04
A375-B1-rep2
A2
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 2312 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 193.78 NGFR rabbit IgG(H+L) / donkey NGFR 179.41
A375-B1-rep2
A3
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 2208 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 193.23 NGFR rabbit IgG(H+L) / donkey NGFR 197.15
A375-B1-rep2
A4
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1701 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 187.13 NGFR rabbit IgG(H+L) / donkey NGFR 188.45
A375-B1-rep2
A5
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 2302 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 198.28 NGFR rabbit IgG(H+L) / donkey NGFR 170.70
A375-B1-rep2
A6
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1975 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 186.12 NGFR rabbit IgG(H+L) / donkey NGFR 191.67
A375-B1-rep2
A7
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 2194 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 176.74 NGFR rabbit IgG(H+L) / donkey NGFR 173.12
A375-B1-rep2
A8
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1958 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 162.65 NGFR rabbit IgG(H+L) / donkey NGFR 176.09
A375-B1-rep2
A9
A-375 Vemurafenib Olaparib 1.0000 0.5333
uM
 48
hr
 2 2162 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 173.14 NGFR rabbit IgG(H+L) / donkey NGFR 197.62
A375-B1-rep2
B2
A-375 Vemurafenib Iniparib 1.0000 0.5333
uM
 48
hr
 2 2392 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 155.20 NGFR rabbit IgG(H+L) / donkey NGFR 165.74
A375-B1-rep2
B3
A-375 Vemurafenib CTB 1.0000 0.5333
uM
 48
hr
 2 2694 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 159.97 NGFR rabbit IgG(H+L) / donkey NGFR 139.51
A375-B1-rep2
B4
A-375 Vemurafenib Rocilinostat 1.0000 0.5333
uM
 48
hr
 2 2904 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 288.39 NGFR rabbit IgG(H+L) / donkey NGFR 128.70
A375-B1-rep2
B5
A-375 Vemurafenib GSK-J1 1.0000 0.5333
uM
 48
hr
 2 2749 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 153.27 NGFR rabbit IgG(H+L) / donkey NGFR 180.25
A375-B1-rep2
B6
A-375 Vemurafenib PFI-1 1.0000 0.5333
uM
 48
hr
 2 2340 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 155.42 NGFR rabbit IgG(H+L) / donkey NGFR 204.08
A375-B1-rep2
B7
A-375 Vemurafenib UNC669 1.0000 0.5333
uM
 48
hr
 2 2288 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 162.78 NGFR rabbit IgG(H+L) / donkey NGFR 184.93
A375-B1-rep2
B8