Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
WM-115 Vemurafenib Pictilisib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 365.63 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 82.20 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 264.89
WM115-P9
F5
WM-115 Vemurafenib AZD8055 0.316 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 653.50 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 78.35 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 490.17
WM115-P9
F6
WM-115 Vemurafenib AZD8055 0.316 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 755.26 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 86.53 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 468.23
WM115-P9
F7
WM-115 Vemurafenib JNK-IN-8 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 308.29 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 84.15 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 475.73
WM115-P9
F8
WM-115 Vemurafenib JNK-IN-8 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 313.62 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 82.91 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 396.60
WM115-P9
F9
WM-115 Vemurafenib Palbociclib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 116.60 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 60.05 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 306.18
WM115-P9
G10
WM-115 Vemurafenib Palbociclib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 60.17 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 56.86 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 304.92
WM115-P9
G11
WM-115 Vemurafenib Saracatinib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 380.80 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 95.99 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 335.40
WM115-P9
G2
WM-115 Vemurafenib Saracatinib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 422.31 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 95.23 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 296.19
WM115-P9
G3
WM-115 Vemurafenib Pictilisib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 385.98 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 86.02 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 343.20
WM115-P9
G4
WM-115 Vemurafenib Pictilisib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 517.76 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 122.59 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 298.64
WM115-P9
G5
WM-115 Vemurafenib AZD8055 1.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 573.82 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 86.77 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 490.85
WM115-P9
G6
WM-115 Vemurafenib AZD8055 1.000 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 787.27 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 118.07 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 448.28
WM115-P9
G7
WM-115 Vemurafenib JNK-IN-8 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 313.29 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 78.88 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 452.14
WM115-P9
G8
WM-115 Vemurafenib JNK-IN-8 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 294.05 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 73.04 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 351.08
WM115-P9
G9