Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
WM-115 Vemurafenib Trametinib 0.000 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 426.47 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 110.31 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 473.36
WM115-P3
E5
WM-115 Vemurafenib Defactinib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 586.58 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 167.91 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 281.34
WM115-P3
E6
WM-115 Vemurafenib Defactinib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 475.64 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 155.71 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 250.67
WM115-P3
E7
WM-115 Vemurafenib PF562271 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 644.33 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 162.03 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 254.66
WM115-P3
E8
WM-115 Vemurafenib PF562271 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 313.91 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 115.64 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 120.95
WM115-P3
E9
WM-115 Vemurafenib Dasatinib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 530.67 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 135.29 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 285.13
WM115-P3
F10
WM-115 Vemurafenib Dasatinib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 524.26 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 135.29 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 308.36
WM115-P3
F11
WM-115 Vemurafenib 0.316
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 575.52 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 119.57 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 507.50
WM115-P3
F2
WM-115 Vemurafenib 0.316
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 613.60 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 129.28 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 566.50
WM115-P3
F3
WM-115 Vemurafenib Trametinib 0.316 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 446.29 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 110.40 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 454.27
WM115-P3
F4
WM-115 Vemurafenib Trametinib 0.316 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 446.09 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 119.13 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 484.62
WM115-P3
F5
WM-115 Vemurafenib Defactinib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 447.13 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 106.30 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 516.28
WM115-P3
F6
WM-115 Vemurafenib Defactinib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 418.11 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 118.21 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 444.82
WM115-P3
F7
WM-115 Vemurafenib PF562271 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 451.63 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 105.14 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 424.95
WM115-P3
F8
WM-115 Vemurafenib PF562271 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 250.63 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 92.47 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 166.78
WM115-P3
F9
WM-115 Vemurafenib Dasatinib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 366.95 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 105.75 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 296.66
WM115-P3
G10
WM-115 Vemurafenib Dasatinib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 476.13 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 138.74 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 340.95
WM115-P3
G11
WM-115 Vemurafenib 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 410.88 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 100.95 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 458.18
WM115-P3
G2
WM-115 Vemurafenib 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 481.16 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 99.95 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 488.57
WM115-P3
G3
WM-115 Vemurafenib Trametinib 1.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 393.03 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 103.89 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 423.19
WM115-P3
G4
WM-115 Vemurafenib Trametinib 1.000 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 468.25 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 114.36 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 450.42
WM115-P3
G5
WM-115 Vemurafenib Defactinib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 399.85 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 95.14 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 453.50
WM115-P3
G6
WM-115 Vemurafenib Defactinib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 358.03 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 90.20 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 412.30
WM115-P3
G7
WM-115 Vemurafenib PF562271 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 423.89 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 108.70 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 368.15
WM115-P3
G8
WM-115 Vemurafenib PF562271 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 320.65 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 105.65 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 173.92
WM115-P3
G9