Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 4 of 4) - Dataset (ID:20277)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein 1 Detected (647) Protein 2 Detected (647) Protein 3 Detected (647) Protein 4 Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
WM-115 Vemurafenib 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 91.71 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 223.95
WM115-P2
C5
WM-115 Vemurafenib 3.160
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 15.97 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 178.14
WM115-P2
C6
A-375 Vemurafenib 0.316
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 179.36 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 772.74
A375-P4
C4
COLO 858 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1478.74 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 103.01
Colo858-P2
C2
COLO 858 Vemurafenib 0.100
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 813.35 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 449.08
Colo858-P2
C3
COLO 858 Vemurafenib 0.316
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 412.31 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 482.64
Colo858-P2
C4
WM-115 Vemurafenib 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 107.90 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 207.30
WM115-P2
B5
WM-115 Vemurafenib 0.316
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 163.26 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 227.60
WM115-P2
B4
WM-115 Vemurafenib 0.100
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 235.84 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 227.32
WM115-P2
B3
WM-115 Vemurafenib 0.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 353.24 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 111.40
WM115-P2
B2
A-375 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1389.99 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 682.68
A375-P1
C2
A-375 Vemurafenib 0.100
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 714.42 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 117.04
A375-P1
C3
A-375 Vemurafenib 0.316
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 382.53 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 61.39
A375-P1
C4
A-375 Vemurafenib 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 185.44 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 49.39
A375-P1
C5
A-375 Vemurafenib 3.160
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 110.57 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 45.79
A375-P1
C6
A-375 Vemurafenib 0.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1328.75 NGFR rabbit IgG(H+L) / donkey NGFR 386.89
A375-P3
B2
COLO 858 Vemurafenib 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 268.36 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 540.81
Colo858-P2
C5
COLO 858 Vemurafenib 3.160
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 209.32 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 478.41
Colo858-P2
C6
A-375 Vemurafenib 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 9.44 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 383.48
A375-P4
C5
A-375 Vemurafenib 3.160
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 44.69 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 464.61
A375-P2
C6
A-375 Vemurafenib 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 149.16 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 450.58
A375-P2
C5
A-375 Vemurafenib 0.316
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 340.94 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 468.39
A375-P2
C4
A-375 Vemurafenib 0.100
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 719.45 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 412.70
A375-P2
C3
A-375 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1315.15 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 172.98
A375-P2
C2
A-375 Vemurafenib 3.160
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -22.64 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 229.55
A375-P4
C6